Cellular Dyes
MitoTracker® Red CMXRos #9082
Directions for Use
Each vial of MitoTracker® Red CMXRos contains 50 μg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 94.1 μl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results are obtained by diluting the stock solution directly into growth media at a concentration between 15-125 nM and incubation between 15-45 min at 37ºC. After incubation, fix the cells in ice-cold methanol for 15 min at -20ºC then rinse 3 times with PBS for 5 min. Proceed with immunostaining.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using MitoTracker® Red CMXRos (red) and Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) #8617 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
MitoTracker® Red CMXRos is well retained after fixation allowing for further sample processing and immunostaining. Excitation: 579 nm, Emission: 599 nm, Molecular Weight: 531.52
Background
MitoTracker® dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety for mitochondrial labeling. To label mitochondria, cells are simply incubated with MitoTracker® probes, which passively diffuse across the plasma membrane and accumulate in active mitochondria.
Application References
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The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. MitoTracker® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
