Product Pathways - Neuroscience
MAG Antibody #9086
Have you tried your application using our XP® monoclonal antibodies? Try product: 9043
PhosphoSitePlus® protein, site, and accession data: MAG
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 100 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9086:
- Western Blotting
Specificity / Sensitivity
MAG Antibody recognizes endogenous levels of total MAG protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu615 of human MAG protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from human cerebellum, human spinal cord, and mouse brain using MAG Antibody.
Background
Myelin-associated glycoprotein (MAG), which contains five immunoglobulin-like domains, is a highly glycosylated protein (1). MAG is a component of all myelinated internodes, whether formed by oligodendrocytes in the central nervous system (CNS) or by Schwann cells in the peripheral nervous system (PNS) (2), and has several functions. A known function of MAG is its inhibition of axonal regeneration after injury. It inhibits axonal outgrowth from adult dorsal root ganglion and in postnatal cerebellar, retinal, spinal, hippocampal, and superior cervical ganglion neurons (3). Interaction between MAG and several other molecules on the innermost wrap of myelin and complementary receptors on the opposing axon surface are required for long-term axon stability. Without MAG, myelin is still expressed, but long-term axon degeneration and altered axon cytoskeleton structure can be seen (4).
- Li, M. et al. (1996) J Neurosci Res 46, 404-14.
- Nguyen, T. et al. (2009) J Neurosci 29, 630-7.
- Yamashita, T. et al. (2002) J Cell Biol 157, 565-70.
- Mehta, N.R. et al. (2010) ACS Chem Neurosci 1, 215-222.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
