Cell Signaling Technology

Product Pathways - Apoptosis

Lamin B1 Antibody #9087

Applications Reactivity Sensitivity MW (kDa) Source
W H M R (Mk) (B) (Dg) (Pg) Endogenous 68, 45 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Dg=Dog  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Lamin B1 Antibody recognizes endogenous levels of total lamin B1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys415 of human lamin B1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Lamin B1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hr), using Lamin B1 Antibody.

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene LMNA. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene LMNB1 is correlated with pathogenesis of the neurological disorder adult-onset leukodystrophy (10).

  1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  2. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
  3. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
  4. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
  5. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
  6. Biamonti, G. et al. (1992) Mol Cell Biol 12, 3499-506.
  7. Lin, F. and Worman, H.J. (1995) Genomics 27, 230-6.
  8. Pollard, K.M. et al. (1990) Mol Cell Biol 10, 2164-75.
  9. Chandler, J.M. et al. (1997) Biochem J 322 ( Pt 1), 19-23.
  10. Padiath, Q.S. et al. (2006) Nat Genet 38, 1114-23.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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