Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Vesicle Trafficking

LAMP1 (D2D11) XP® Rabbit mAb #9091

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H Mk Endogenous 42 (non-glycosylated), 90-120 (glycosylated) Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LAMP1 (D2D11) XP® Rabbit mAb recognizes endogenous levels of total LAMP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using LAMP1 (D2D11) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using LAMP1 (D2D11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6).

  1. Eskelinen, E.L. et al. (2003) Trends Cell Biol 13, 137-45.
  2. Fukuda, M. (1991) J Biol Chem 266, 21327-30.
  3. Kundra, R. and Kornfeld, S. (1999) J Biol Chem 274, 31039-46.
  4. Rohrer, J. et al. (1996) J Cell Biol 132, 565-76.
  5. Huynh, K.K. et al. (2007) EMBO J 26, 313-24.
  6. Eskelinen, E.L. et al. (2004) Mol Biol Cell 15, 3132-45.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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