Product Pathways - CD Markers
DC-SIGN Antibody #9097
|9097S||100 µl (10 western blots)||---||In Stock||---|
|9097||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Monkey.
Specificity / Sensitivity
DC-SIGN Antibody recognizes endogenous levels of total DC-SIGN protein. This antibody also detects DC-SIGNR.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val134 of human DC-SIGN protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from MUTZ-3 cells, undifferentiated (-) or differentiated (+) into interstitial dendritic cells with Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) #8922 (100 ng/mL), Human Interleukin-4 (hIL-4) #8919 (100 ng/mL), and Human Tumor Necrosis Factor-α (hTNF-α) #8902 (2.5 ng/mL) for 8 d, using DC-SIGN Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from human monocyte-derived dendritic cells using DC-SIGN Antibody.
Western blot analysis of extracts from 293T cells, mock transfected (-), transfected with a construct expressing Myc/DDK-tagged full-length human DC-SIGN (hDC-SIGN-Myc/DDK; +), or transfected with a construct expressing full-length human DC-SIGNR (hDC-SIGNR; +), using DC-SIGN Antibody.
DC-SIGN (CD209, CLEC4L) is a C-type lectin receptor expressed by dendritic cells (DCs) (1,2). The DC-SIGN transcript can undergo several splicing events to generate at least thirteen different transmembrane and soluble isoforms (3). DC-SIGN responds to a broad range of pathogens due to its ability to recognize both mannose and fructose carbohydrates, and is well studied for its role in HIV infection. Recognition of the HIV envelope glycoprotein gp120 by DC-SIGN leads to internalization of HIV by DCs and facilitates transmission of the virus to CD4+ T cells (2,4). DC-SIGN also mediates adhesion to T cells through interaction with ICAM-3, as well as transmigration across the endothelium by binding to ICAM-2 (1,5). The DC-SIGN receptor can modulate TLR signaling by activating the kinase Raf-1 (6,7). The closely related molecule DC-SIGNR (L-SIGN, CLEC4M) is 77% homologous to DC-SIGN and likely arose through a gene duplication event (8). Like DC-SIGN, DC-SIGNR binds mannose carbohydrates on the surface of pathogens (8,9). However, the expression patterns of the two receptors differ, as DC-SIGNR expression is restricted to endothelial cells of the liver, lymph node, and placenta (10). Murine cells contain a set of related molecules, SIGNR1-SIGNR8 (11). Based on sequence analysis, there is no clear murine ortholog to human DC-SIGN, however SIGNR3 is the most functionally similar due to its ability to recognize both mannose and fructose structures (11).
- Geijtenbeek, T.B. et al. (2000) Cell 100, 575-85.
- Geijtenbeek, T.B. et al. (2000) Cell 100, 587-97.
- Mummidi, S. et al. (2001) J Biol Chem 276, 33196-212.
- Kwon, D.S. et al. (2002) Immunity 16, 135-44.
- Geijtenbeek, T.B. et al. (2000) Nat Immunol 1, 353-7.
- Gringhuis, S.I. et al. (2007) Immunity 26, 605-16.
- Gringhuis, S.I. et al. (2010) Nat Immunol 11, 419-26.
- Bashirova, A.A. et al. (2001) J Exp Med 193, 671-8.
- Mitchell, D.A. et al. (2001) J Biol Chem 276, 28939-45.
- Pöhlmann, S. et al. (2001) Proc Natl Acad Sci U S A 98, 2670-5.
- Powlesland, A.S. et al. (2006) J Biol Chem 281, 20440-9.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.