Product Pathways - MAPK Signaling
p44/42 MAPK (Erk1/2) Antibody #9102
|W IP IHC-P IF-IC F||H M R Hm Mk Mi Z B Pg Sc||Endogenous||42, 44||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey Mi=Mink Z=Zebrafish B=Bovine Pg=Pig Sc=S. cerevisiae
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
p44/42 MAPK (Erk1/2) Antibody detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, this antibody recognizes p44 MAPK more readily than p42 MAPK. The antibody does not recognize either JNK/SAPK or p38 MAP kinase.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence in the C-terminus of rat p44 MAP Kinase. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from HeLa cells transfected with 100 nM control siRNA #6201 (-) or p44 MAPK (Erk1) siRNA (+), using p44/42 MAPK (Erk1/2) Antibody #9102 and GCK Antibody #3782. The Erk1/2 antibody confirms silencing of Erk1 expression, and GCK Antibody is used to control for loading and specificity of p44 MAPK (Erk1) siRNA.
Western blot analysis of extracts from serum-induced PC12 cells, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101 (upper) or control p44/42 MAPK (Erk1/2) Antibody (lower).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p44/42 MAPK (Erk1/2) Antibody.
Flow cytometric analysis of Jurkat cells, using p44/42 MAPK (Erk1/2) Antibody (blue) compared to a nonspecific negative control antibody (red).
Confocal immunofluorescent images of C6 cells serum-starved (left) and serum-treated (center), labeled with p44/42 MAPK (Erk1/2) Antibody (green) compared to an isotype control (right). Actin filaments have been labeled with Alex Fluor® 555 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.
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- Fei, Z. et al. (2011) J Biol Chem 286, 27761-8. Applications: Western Blotting
- Wu, M. et al. (2009) Diabetes Metab Res Rev 25, 279-86. Applications: Western Blotting
- Abe, T. et al. (2007) J Virol 81, 8953-66. Applications: Western Blotting
- Yu, C. et al. (2002) Cancer Res 62, 188-99. Applications: Western Blotting
- Rosenberger, C.M. and Finlay, B.B. (2002) J Biol Chem 277, 18753-62. Applications: Western Blotting
- Wu, J. et al. (2002) J Biol Chem 277, 19498-505. Applications: Western Blotting
- Domina, A.M. et al. (2000) J Biol Chem 275, 21688-94. Applications: Western Blotting
- Hayne, C. et al. (2000) J Biol Chem 275, 31876-82. Applications: Western Blotting
- Tan, J. et al. (2000) J Neurosci 20, 7587-94. Applications: Western Blotting
- Dai, Y. et al. (2001) Cancer Res 61, 5106-15. Applications: Western Blotting
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- 9194 p44/42 MAPK (Erk1/2) Control Cell Extracts
- 4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb
- 4376 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb
- 4377 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb
- 9101 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody
- 9106 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb
- 4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
- 4696 p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb
- 4372 p44 MAP Kinase (Erk1) Antibody
- 9107 p44/42 MAPK (Erk1/2) (3A7) Mouse mAb
- 9108 p42 MAP Kinase (Erk2) Antibody
- 8103 SignalSlide® Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Controls
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 5425 Normal Goat Serum
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
For Research Use Only. Not For Use In Diagnostic Procedures.