Product Pathways - Cell Cycle / Checkpoint
PhosphoPlus® cdc2 (Tyr15) Antibody Kit #9110
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|---|---|
| Phospho-cdc2 (Tyr15) Antibody # 9111 | 0.1 milliliters | W IP E-P | H M R Mk Sc Dm X | 34 | Rabbit |
| cdc2 Antibody # 9112 | 0.1 milliliters | W | H M R | 34 | Rabbit |
| cdc2 (Tyr15) Control Proteins # 9113 | 40 microliters | 32, 34 | |||
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 50 microliters | Goat | |||
| Anti-biotin, HRP-linked Antibody # 7075 | 0.1 milliliters | Goat | |||
| Biotinylated Protein Ladder Detection Pack # 7727 | 0.1 milliliters | ||||
| 20X LumiGLO® Reagent and 20X Peroxide # 7003 | 5 milliliters each | ||||
| cdc2 (Tyr15) Control Proteins # 9113 | 80 microliters | 32, 34 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
E-P=ELISA (Peptide)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Sc=S. cerevisiae
Dm=D. melanogaster
X=Xenopus
Specificity / Sensitivity
When used in conjunction with our Phototope®-HRP Western Detection Kit, Phospho-cdc2 (Tyr15) Antibody detects less than 10 ng of tyrosine phosphorylated cdc2 yet will not react with up to 1 µg of non-tyrosine phosphorylated cdc2. Similarly, Phospho-cdc2 (Tyr15) Antibody detects phosphorylated tyrosine 15 of cdk2 but demonstrates no cross reactivity to cdk4, cdk6 and cdk7 or other phospho-tyrosine proteins.
Western Blotting
Western analysis of extracts from hydroxyurea (G1/S) or nocodizate (G2/M) treated Saos cells with Phospho-cdc2 (Tyr15) (A) and cdc2 (Tyr15) Antibodies (B).
Table
Role of cdc2 Y15 phosporylation in the progression of eukaryotic cells into M-phase of the cell cycle.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to residues surrounding the phsphorylation site. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Tyr15 and Thr14 (2). Phosphorylation at Thr14 and Tyr15 resulting in inhibition of cdc2 can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).
- Atherton-Fessler, S. et al. (1994) Mol. Biol. Cell. 5, 989-1001.
- Norbury, C. et al. (1991) EMBO. J. 10, 3321-3329.
- McGowan, C.H. and Russell, P. (1993) EMBO J. 12, 75-85.
- Wells, N.J. et al. (1999) J. Cell. Sci. 112, 3361-3371.
- Hunter, T. (1995) Cell 80, 225-236.
Application References
- Yu, C. et al. (2002) Pharmacologic mitogen-activated protein/extracellular signal-regulated kinase kinase/mitogen-activated protein kinase inhibitors interact synergistically with STI571 to induce apoptosis in Bcr/Abl-expressing human leukemia cells. Cancer Res. 62, 188-199. This article references the use of PhosphoPlus® cdc2 (Tyr15) Antibody Kit in the following applications: Western Blotting
- Dai, Y. et al. (2001) Pharmacological inhibitors of the mitogen-activated protein kinase (MAPK) kinase/MAPK cascade interact synergistically with UCN-01 to induce mitochondrial dysfunction and apoptosis in human leukemia cells. Cancer Res. 61, 5106-5115. This article references the use of PhosphoPlus® cdc2 (Tyr15) Antibody Kit in the following applications: Western Blotting
- Misaki, K. et al. (2001) PKN delays mitotic timing by inhibition of Cdc25C: possible involvement of PKN in the regulation of cell division. Proc. Natl. Acad. Sci. USA 98, 125-129. This article references the use of PhosphoPlus® cdc2 (Tyr15) Antibody Kit in the following applications: Western Blotting
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