Product Pathways - Cell Cycle / Checkpoint
PhosphoPlus® cdc2 (Tyr15) Antibody Kit #9110
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| Phospho-cdc2 (Tyr15) Antibody #9111 | 100 µl | W IP | H M R Mk Dm X | 34 | Rabbit |
| cdc2 Antibody #9112 | 100 µl | W | H M R | 34 | Rabbit |
| cdc2 (Tyr15) Control Proteins #9113 | 100 µl | 32, 34 | |||
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat | |||
| Anti-biotin, HRP-linked Antibody #7075 | 100 µl | Goat | |||
| Biotinylated Protein Ladder Detection Pack #7727 | 100 µl | ||||
| 20X LumiGLO® Reagent and 20X Peroxide #7003 | 5 ml each |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Dm=D. melanogaster
X=Xenopus
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
When used in conjunction with our Phototope®-HRP Western Detection Kit, Phospho-cdc2 (Tyr15) Antibody detects less than 10 ng of tyrosine phosphorylated cdc2 yet will not react with up to 1 µg of non-tyrosine phosphorylated cdc2. Similarly, Phospho-cdc2 (Tyr15) Antibody detects phosphorylated tyrosine 15 of cdk2 but demonstrates no cross reactivity to cdk4, cdk6 and cdk7 or other phospho-tyrosine proteins.
Western Blotting
Western analysis of extracts from hydroxyurea (G1/S) or nocodizate (G2/M) treated Saos cells with Phospho-cdc2 (Tyr15) (A) and cdc2 (Tyr15) Antibodies (B).
Description
PhosphoPlus® Cdc2 (Tyr15) Western Detection Kit offers an efficient way of detecting cdc2 (Tyr15) phosphorylation by western blotting. The kit contains enough primary and secondary antibodies to perform 10 western blots, as well as a set of pre-stained and biotinylated markers, control proteins and LumiGLO® reagent.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the phsphorylation site. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).
- Atherton-Fessler, S. et al. (1994) Mol. Biol. Cell. 5, 989-1001.
- Norbury, C. et al. (1991) EMBO. J. 10, 3321-3329.
- McGowan, C.H. and Russell, P. (1993) EMBO J. 12, 75-85.
- Wells, N.J. et al. (1999) J. Cell. Sci. 112, 3361-3371.
- Hunter, T. (1995) Cell 80, 225-236.
Application References
- Yu, C. et al. (2002) Pharmacologic mitogen-activated protein/extracellular signal-regulated kinase kinase/mitogen-activated protein kinase inhibitors interact synergistically with STI571 to induce apoptosis in Bcr/Abl-expressing human leukemia cells. Cancer Res. 62, 188-199. Applications: Western Blotting
- Dai, Y. et al. (2001) Pharmacological inhibitors of the mitogen-activated protein kinase (MAPK) kinase/MAPK cascade interact synergistically with UCN-01 to induce mitochondrial dysfunction and apoptosis in human leukemia cells. Cancer Res. 61, 5106-5115. Applications: Western Blotting
- Misaki, K. et al. (2001) PKN delays mitotic timing by inhibition of Cdc25C: possible involvement of PKN in the regulation of cell division. Proc. Natl. Acad. Sci. USA 98, 125-129. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Protocols
- 9111:
- ELISA Peptide, Immunoprecipitation, Western Blotting
- 9112:
- Western Blotting
Companion Products
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 9114 Phospho-cdc2 (Thr161) Antibody
- 9116 cdc2 (POH1) Mouse mAb
- 9111 Phospho-cdc2 (Tyr15) Antibody
- 9113 cdc2 (Tyr15) Control Proteins
For Research Use Only. Not For Use In Diagnostic Procedures.
