Product Pathways - Cell Cycle / Checkpoint
Phospho-cdc2 (Thr161) Antibody #9114
|9114S||100 µl (10 western blots)||---||In Stock||---|
|9114||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||34||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Phospho-cdc2 (Thr161) Antibody detects endogenous levels of cdc2 only when phosphorylated at threonine 161. The antibody cross-reacts with endogenous CDK2 phosphorylated at threonine 160.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr161 of human cdc2. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of cdc2 kinase treated with lambda protein phosphatase (lambda-PPase) for the indicated times, using Phospho-cdc2 (Thr161) Antibody.
The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).
cdc2 activation and association with cyclin A require phosphorylation at Thr161 by the CDK-activating kinase CAK, a complex of CDK7 and cyclin H (7,8).
- Atherton-Fessler, S. et al. (1994) Mol. Biol. Cell. 5, 989-1001.
- Norbury, C. et al. (1991) EMBO. J. 10, 3321-3329.
- McGowan, C.H. and Russell, P. (1993) EMBO J. 12, 75-85.
- Wells, N.J. et al. (1999) J. Cell. Sci. 112, 3361-3371.
- Hunter, T. (1995) Cell 80, 225-236.
- Fesquet, D. et al. (1993) EMBO J. 12, 3111-3121.
- Ducommun, B. et al. (1991) EMBO J. 10, 3311-3319.
- De Smedt, V. et al. (2002) J. Biol. Chem. 277, 28592-28600. Applications: Western Blotting.
- Baus, F. et al. (2003) EMBO J. 22, 3992-4002. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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