Product Pathways - Lymphocyte Signaling

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Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb #9115

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IF-IC	H M (Mk)	Endogenous	95-100	Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

9115:

Immunofluorescence, Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb detects endogenous levels of total Blimp-1/PRDI-BF1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val90 of human PRDI-BF1.

Background

Blimp-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor that is considered a master regulator of terminal B-cell development (1). The human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-β promoter and can inhibit virus-mediated IFN-β production (2). Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1 (1). In the B-cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation (3) and in a subset of T-cells (4,5) suggesting it may play a wider role in homeostasis and differentiation (6). Mechanistically, Blimp-1 is thought to act by recruiting chromatin-modifying enzymes including histone deacetylases (7) and methyltransferases (8,9). Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc (10), CIITA (11), Pax5 (12), Spi-B, and Id3 (13).

1. Turner, C.A. et al. (1994) Cell 77, 297-306.
2. Keller, A.D. and Maniatis, T. (1991) Genes Dev 5, 868-79.
3. Chang, D.H. et al. (2000) Nat Immunol 1, 169-76.
4. Kallies, A. et al. (2006) Nat Immunol 7, 466-74.
5. Martins, G.A. et al. (2006) Nat Immunol 7, 457-65.
6. Kallies, A. and Nutt, S.L. (2007) Curr Opin Immunol 19, 156-62.
7. Yu, J. et al. (2000) Mol Cell Biol 20, 2592-603.
8. Gyory, I. et al. (2004) Nat Immunol 5, 299-308.
9. Ancelin, K. et al. (2006) Nat Cell Biol 8, 623-30.
10. Lin, Y. et al. (1997) Science 276, 596-9.
11. Chen, H. et al. (2007) Mol Immunol 44, 1461-70.
12. Lin, K.I. et al. (2002) Mol Cell Biol 22, 4771-80.
13. Shaffer, A.L. et al. (2006) Immunol Rev 210, 67-85.

Application References

• Smith, M.A. et al. (2010) J Immunol 185, 6058-67. Applications: ChIP Western Blotting
• Desai, S. et al. (2010) Mol Cancer Res 8, 907-18. Applications: Western Blotting
• Cubedo, E. et al. (2011) FEBS J 278, 3065-75. Applications: ChIP

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

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For Research Use Only. Not For Use In Diagnostic Procedures.