Product Pathways - Lymphocyte Signaling
Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb #9115
|9115S||100 µl (10 western blots)||---||In Stock||---|
|9115||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||95-100||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Species predicted to react based on 100% sequence homology: Monkey.
Specificity / Sensitivity
Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb detects endogenous levels of total Blimp-1/PRDI-BF1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val90 of human PRDI-BF1.
Western blot analysis of extracts from Karpas 620, SR and H929 cell lines using Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb.
Blimp-1 (B lymphocyte-induced maturation protein) is a nuclear zinc-finger containing transcriptional repressor that is considered a master regulator of terminal B-cell development (1). The human homolog, PRDI-BF1, was identified by its ability to bind to the PRDI element on the IFN-β promoter and can inhibit virus-mediated IFN-β production (2). Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1 (1). In the B-cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation (3) and in a subset of T-cells (4,5) suggesting it may play a wider role in homeostasis and differentiation (6). Mechanistically, Blimp-1 is thought to act by recruiting chromatin-modifying enzymes including histone deacetylases (7) and methyltransferases (8,9). Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc (10), CIITA (11), Pax5 (12), Spi-B, and Id3 (13).
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- Smith, M.A. et al. (2010) J Immunol 185, 6058-67. Applications: Western Blotting, Chromatin IP.
- Desai, S. et al. (2010) Mol Cancer Res 8, 907-18. Applications: Western Blotting.
- Cubedo, E. et al. (2011) FEBS J 278, 3065-75. Applications: Chromatin IP.
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For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.