Cell Signaling Technology

Product Pathways - Apoptosis

Maspin (L250) Antibody #9119

Applications Reactivity Sensitivity MW (kDa) Source
W H (Mk) Endogenous 40 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Maspin (Q260) Antibody detects endogenous levels of total maspin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu250 of human maspin. Antibodies were purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-1080, HT-29 and COLO-201 cell lines using Maspin (L250) Antibody.

Background

Maspin (SERPINB5) was discovered as a mammary tumor suppressor that is expressed in normal mammary epithelium but lost in most breast cancer cell lines (1). While maspin is related to the serpin family of serine protease inhibitors, it may not function as a protease inhibitor (2). It plays an essential role in embryonic development through critical roles in cell adhesion (3). While the precise mechanism of maspin signaling is unclear (4), the tumor suppressing activity of maspin has been attributed to its ability to inhibit cell invasion/metastasis (5,6) and angiogenesis (7), while promoting apoptosis (8). Nuclear translocation of active IKKα has been shown to repress maspin transcription and promote prostate cancer metastasis (9).

  1. Zou, Z. et al. (1994) Science 263, 526-9.
  2. Pemberton, P.A. et al. (1995) J Biol Chem 270, 15832-7.
  3. Gao, F. et al. (2004) Development 131, 1479-89.
  4. Bailey, C.M. et al. (2006) J Cell Physiol 209, 617-24.
  5. Sheng, S. et al. (1996) Proc Natl Acad Sci USA 93, 11669-74.
  6. Seftor, R.E. et al. (1998) Cancer Res 58, 5681-5.
  7. Zhang, M. et al. (2000) Nat Med 6, 196-9.
  8. Jiang, N. et al. (2002) Oncogene 21, 4089-98.
  9. Luo, J.L. et al. (2007) Nature 446, 690-4.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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