Product Pathways - MAPK Signaling
MEK1/2 Antibody #9122
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IP | H M R Mk Dr X | 45 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Dr=Drosophila
X=Xenopus
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
MEK1/2 Antibody detects endogenous levels of total MEK1/2 protein. The antibody does not cross-react with related kinases such as SEK (MKK4), MKK3 or MKK6.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) derived from the sequence of human MEK1/2. Antibodies are purified by protein A and peptide affinity chromatography.
Background
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221 (in the activation loop of subdomain VIII) by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
- Crews, C.M. et al. (1992) Science 258, 478-480.
- Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
- Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
- Cowley, S. et al. (1994) Cell 77, 841-852.
Application References
- Bueno, O. F. et al. (2000) The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice. European Molecular Biology Organization (EMBO) 19, 6341-6350. This article references the use of MEK1/2 Antibody in the following applications: Western Blotting
- Umenishi, F. and Schrier, R.W. (2003) Hypertonicity-induced aquaporin-1 (AQP1) expression is mediated by the activation of MAPK pathways and hypertonicity-responsive element in the AQP1 gene. J. Biol. Chem. 278, 15765-15770. This article references the use of MEK1/2 Antibody in the following applications: Western Blotting
- Piatelli, M. J. et al. (2004) Phosphatidylinositol 3-kinase-dependent mitogen-activated protein/extracellular signal-regulated kinase kinase 1/2 and NF-kappa B signaling pathways are required for B cell antigen receptor-mediated cyclin D2 induction in mature B cells. J. Immunol. 172, 2753-2762. This article references the use of MEK1/2 Antibody in the following applications: Western Blotting
- Rosenberger, C.M. and Finlay, B.B. (2002) Macrophages Inhibit Salmonella Typhimurium Replication through MEK/ERK Kinase and Phagocyte NADPH Oxidase Activities. The Journal of Biological Chemistry 277, 18753-18762. This article references the use of MEK1/2 Antibody in the following applications: Western Blotting
- Deszo, E. L. et al. (2001) Cd45 negatively regulates monocytic cell differentiation by inhibiting phorbol 12-myristate13-acetate-dependent activation and tyrosine phosphorylation of C70protein kinase cdelta. Journal of Biological Chemistry 276, 10212-10217. This article references the use of MEK1/2 Antibody in the following applications: Western Blotting
- Furuno, T. et al. (2001) Nuclear shuttling of mitogen-activated protein (map) kinase (extracellular signal-regulated kinase (erk) 2) was dynamically controlled by map/erk kinase after antigen stimulation inrbl-2h3 cells. Journal of Immunology 166, 4416-4421. This article references the use of MEK1/2 Antibody in the following applications: IC-IF Western Blotting
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