Product Pathways - MAPK Signaling
MEK1/2 (47E6) Rabbit mAb #9126
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IF-IC F | H M R Mk | 45 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
MEK1/2 (47E6) Rabbit mAb detects endogenous levels of total MEK1/2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human MEK1/2.
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, COS and PC12 cells, using MEK1/2 (47E6) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using MEK1/2 Antibody compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent images of untreated HeLa cells labeled with MEK1/2 (47E6) Rabbit mAb (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221 (in the activation loop of subdomain VIII) by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
- Crews, C.M. et al. (1992) Science 258, 478-480.
- Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
- Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
- Cowley, S. et al. (1994) Cell 77, 841-852.
Application References
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Companion Products
- 9120 PhosphoPlus® MEK1/2 (Ser217/221) Antibody Kit
- 9121 Phospho-MEK1/2 (Ser217/221) Antibody
- 9122 MEK1/2 Antibody
- 9124 MEK1 Antibody
- 9125 MEK2 Antibody
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- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
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- 9128 Phospho-MEK1 (Ser298) Antibody
- 9160 MEK1/2 HeLa Control Cell Extracts
- 9146 MEK1 (30C8) Rabbit mAb
- 9147 MEK2 (13E3) Rabbit mAb
- 9154 Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
- 2338 Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb
- 2352 MEK1 (61B12) Mouse mAb
- 4694 MEK1/2 (L38C12) Mouse mAb
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.