Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

PhosphoPlus® Stat3 (Tyr705) Antibody Kit #9130

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
Stat3 Antibody #9132 100 µl W IP IHC-P ChIP H M R Mk (B) 79, 86 Rabbit
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 100 µl W IP IHC-P IHC-F IF-IC F ChIP H M R Mk (Hm) (B) (Pg) (Hr) 79, 86 Rabbit IgG
Stat3 Control Cell Extracts #9133 40 µl Human
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat
Anti-biotin, HRP-linked Antibody #7075 100 µl Goat
Biotinylated Protein Ladder Detection Pack #7727 100 µl
20X LumiGLO® Reagent and 20X Peroxide #7003 5 ml

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry  ChIP=Chromatin IP
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  B=Bovine  Pg=Pig  Hr=Horse
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Phospho-Stat3 (Tyr705) Antibody detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. The antibody does not cross-react with other Stat proteins when phosphorylated on the corresponding tyrosine residue, but does cross-react with EGFR. Stat3 antibody detects endogenous levels of total Stat3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from IFN-α stimulated HeLa cells alone or with λ phosphatase treatment using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (left) or Stat3 Antibody #9132 (right).

Description

Source / Purification

Polyclonal antibodies to Phospho-Stat3 are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3 (Phospho-Stat3 (Tyr705) Antibody), or with a synthetic peptide corresponding to mouse Stat3 (Stat3 antibody). Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

Application References

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Protocols

* Product-specific protocol.

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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