Product Pathways - Jak/Stat Pathway
PhosphoPlus® Stat3 (Tyr705) Antibody Kit #9130
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|---|---|
| Phospho-Stat3 (Tyr705) Antibody # 9131 | 100 microliters | W IP IF-IC | H M R C (B) | 79, 86 | Rabbit |
| Stat3 Antibody # 9132 | 100 microliters | W IP IHC-P | H M R (B) | 79, 86 | Rabbit |
| Stat3 Control Cell Extracts # 9133 | 40 microliters | Human | |||
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 50 microliters | Goat | |||
| Anti-biotin, HRP-linked Antibody # 7075 | 100 microliters | Goat | |||
| 20X LumiGLO® Reagent and 20X Peroxide # 7003 | 5 milliliters | ||||
| Biotinylated Protein Ladder Detection Pack # 7727 | 100 microliters |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
C=Chicken
B=Bovine
Specificity / Sensitivity
Phospho-Stat3 (Tyr705) Antibody detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. The antibody does not cross-react with other Stat proteins when phosphorylated on the corresponding tyrosine residue, but does cross-react with EGFR. Stat3 antibody detects endogenous levels of total Stat3 protein.
Western Blotting
Western analysis of extracts from CNTF-treated SK-N-MC cells and EGF-treated A431 cells using Phospho-Stat3 (Tyr705) (A) or Stat3 Antibodies (B).
IP
Immunoprecipitation of 35S-labeled SK-N-MC cell extracts treated with CNTF using Phospho-Stat3 (Tyr705) or Stat3 Antibodies.
IHC-FL (floating)
Confocal images of immunostaining with Phospho-Stat3 (Tyr705) Antibody (green) and propidium iodide (red) in rat dentate gyrus regions from tissue sections of control and 15 minute transient cerebral ischemia followed by 30 minutes of reperfusion. Yellow represents overlay of red and green. (Provided by Dr. Bingren Hu, Queen's Medical Center, Hawaii).
IC-ABC
Immunocytochemistry: Phospho-Stat3 (Tyr705) and Stat3 Antibodies allows in situ detection of IL-6-induced Stat3 nuclear translocation in NIH-3T3 cells.
Source / Purification
Polyclonal antibodies to Phospho-Stat3 are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr705 of mouse Stat3 (Phospho-Stat3 (Tyr705) Antibody), or with a synthetic peptide derived from mouse Stat3 (Stat3 antibody). Antibodies are purified by protein A and peptide affinity chromatography.
Background
The Stat3 transcription factor is an important signaling molecule for many cytokines and growth-factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
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Application References
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