Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat3 Control Cell Extracts #9133

Description

Nonphosphorylated Stat3 Cell Extracts: Total cell extracts from serum-starved HeLa cells prepared without treatment, serve as a negative control. Supplied in SDS Sample Buffer. Store at -20ºC.Phosphorylated Stat3 Cell Extracts: Total cell extracts from serum-starved HeLa cells prepared with IFN-a treatment, serve as a positive control. Supplied in SDS Sample Buffer. Store at -20ºC.

Applications

CST recommends using 10 µl of phosphorylated and nonphosphorylated Stat3 extracts as controls.Note: These lysates are useful for Phospho-Stat3 (Tyr705) Antibody #9131, Phospho-Stat3 (Tyr705) (D3A7) #9145, Phospho-Stat3 (Tyr705) (3E2) #9138 and Phospho-Stat1 (Tyr701) Antibody #9171. However, they are not useful for Phospho-Stat3 (Ser727) Antibody #9134.

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

Application References

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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