Product Pathways - Jak/Stat Pathway

Phospho-Stat3 (Ser727) Antibody #9134

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Applications	Reactivity	MW (kDa)	Source
W IP IF-IC	H M R (B)	86	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Stat3 (Ser727) Antibody detects endogenous levels of Stat3 only when phosphorylated at Ser727. The antibody does not significantly cross-react with the corresponding phospho-serines of other Stat proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser727 of mouse Stat3. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth-factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

1. Heim, M.H. (1999) J. Recept. Signal Transduct. Res. 19, 75-120.
2. Takeda, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 3801-3804.
3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-115.
4. Garcia, R. and Jove, R. (1998) J. Biomed. Sci. 5, 79-85.
5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
6. Darnell Jr., J.E. et al. (1994) Science 264, 1415-1421.
7. Ihle, J.N. (1995) Nature 377, 591-594.
8. Wen, Z. et al. (1995) Cell 82, 241-250.
9. Yokogami, K. et al. (2000) Curr. Biol. 10, 47-50.
10. Biethahn, S. et al. (1999) Exp. Hematol. 27, 885-894.

Application References

• Bromberg, J. F. et al. (1999) Stat3 as an oncogene. Cell 98, 295-303. This article references the use of Phospho-Stat3 (Ser727) Antibody in the following applications: Western Blotting
• Wu, Y.Y. and Bradshaw, R.A. (2000) Activation of the Stat3 signaling pathway is required for differentiation by interleukin-6 in PC12-E2 cells. J. Biol. Chem. 275, 2147-2156. This article references the use of Phospho-Stat3 (Ser727) Antibody in the following applications: Western Blotting
• Teague, T. K. et al. (2000) Activation-induced inhibition of interleukin 6-mediated T cell survival and signal transducer and activator of transcription 1 signaling. J. Exp. Med. 191, 915-926. This article references the use of Phospho-Stat3 (Ser727) Antibody in the following applications: Western Blotting
• Matsuda, T. et al. (1999) STAT3 activation is sufficient to maintain an undifferentiated state of mouse embryonic stem cells. EMBO J. 18, 4261-4269. This article references the use of Phospho-Stat3 (Ser727) Antibody in the following applications: Western Blotting
• Schuringa, J. J. et al. (2001) MEN2A-RET-induced cellular transformation by activation of STAT3. Oncogene 20 (38), 5350-5358. This article references the use of Phospho-Stat3 (Ser727) Antibody in the following applications: Western Blotting
• Simon, A. R. et al. (2000) Regulation of STAT3 by direct binding to the rac1 GTPase. Science 290, 144-147. This article references the use of Phospho-Stat3 (Ser727) Antibody in the following applications: Western Blotting

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