Product Pathways - Neuroscience
Phospho-TrkA (Tyr490) Antibody #9141
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | R (H) (M) | Endogenous | 140 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-TrkA (Tyr490) Antibody detects endogenous levels of Trk only when phosphorylated at tyrosine 490. This antibody also detects TrkB and TrkC when phosphorylated at the corresponding residues.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr490 of human TrkA. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from PC12 cells, untreated or NGF-treated (100 ng/ml), using Phospho-TrkA (Tyr490) Antibody.
Background
The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1).
- Pierotti, M.A. and Greco, A. (2006) Cancer Lett. 232, 90-98.
- Segal, R.A. and Greenberg, M.E. (1996) Annu. Rev. Neurosci. 19, 463-489.
- Stephens, R.M. et al. (1994) Neuron 12, 691-705.
- Obermeier, A. et al. (1993) EMBO J. 12, 933-941.
- Obermeier, A. et al. (1994) EMBO J. 13, 1585-1590.
- Yao, R. and Cooper, G.M. (1995) Science 267, 2003-2006.
Application References
- Anneren, C. et al. (2000) GTK, a Src-related tyrosine kinase, induces nerve growth factor-independent neurite outgrowth in PC12 cells through activation of the Rap1 pathway. J. Biol. Chem. 275, 29153-29161. This article references the use of Phospho-TrkA (Tyr490) Antibody in the following applications: Western Blotting
- Binder, D. K. et al. (1999) Immunohistochemical evidence of seizure-induced activation of trk receptors in the mossy fiber pathway of adult rat hippocampus. J. Neurosci. 19, 4616-4626. This article references the use of Phospho-TrkA (Tyr490) Antibody in the following applications: IHC-FL (floating/frozen) Western Blotting
- Gil, C. et al. (2000) Activation of signal transduction pathways involving trkA, PLCgamma-1, PKC isoforms and ERK-1/2 by tetanus toxin. FEBS Lett. 481, 177-182. This article references the use of Phospho-TrkA (Tyr490) Antibody in the following applications: Western Blotting
- Reuther, G. W. et al. (2000) Identification and characterization of an activating TrkA deletion mutation in acute myeloid leukemia. Mol. Cell. Biol. 20, 8655-8666. This article references the use of Phospho-TrkA (Tyr490) Antibody in the following applications: Western Blotting
- Binder, D. K. et al. (1999) Selective inhibition of kindling development by intraventricular administration of TrkB receptor body. J. Neurosci. 19, 1424-1436. This article references the use of Phospho-TrkA (Tyr490) Antibody in the following applications: Western Blotting
- Xu, B. et al. (2002) Neuroscience 115, 1295-308. This article references the use of Phospho-TrkA (Tyr490) Antibody in the following applications: Western Blotting
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
