Product Pathways - MAPK Signaling
MEK1 (30C8) Rabbit mAb #9146
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | H M R Mk | 45 | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
MEK1 (30C8) Rabbit mAb detects endogenous levels of total MEK1 protein. This antibody does not cross-react with MEK2 and other MAPK kinases.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to human MEK1.
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3 and COS cells, using MEK1 (30C8) Rabbit mAb.
Background
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221 (in the activation loop of subdomain VIII) by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
- Crews, C.M. et al. (1992) Science 258, 478-480.
- Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
- Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
- Cowley, S. et al. (1994) Cell 77, 841-852.
Application References
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- 9121 Phospho-MEK1/2 (Ser217/221) Antibody
- 9127 Phospho-MEK1 (Thr286) Antibody
- 9128 Phospho-MEK1 (Ser298) Antibody
- 9147 MEK2 (13E3) Rabbit mAb
- 2338 Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
