Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

SMG-1 (D42D5) Rabbit mAb #9149

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 410 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SMG-1 (D42D5) Rabbit mAb recognizes endogenous levels of total SMG-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SMG-1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from U-2 OS, 293, and MCF7 cells using SMG-1 (D42D5) Rabbit mAb.

IP

IP

Immunoprecipitation of SMG-1 from U-2 OS cells using SMG-1 (D42D5) Rabbit mAb. Western blot was performed using the same antibody. Lane 1 is 5% input.

Background

SMG-1 is a member of the phosphoinositide 3-kinase-related kinase (PIKK) family, which includes ATM, ATR, mTOR, DNA-PKcs, and TRRAP (1,2). Activated by DNA damage, SMG-1 has been shown to phosphorylate p53 and hUpf1 (SMG-2) (1-4). hUpf1 is a subunit of the surveillance complex that allows degradation of messenger RNA species containing premature termination codons (PTCs). This process, known as nonsense-mediated mRNA decay (NMD), prevents the translation of truncated forms of proteins that may result in gain of function or dominant negative species. NMD occurs under normal cellular conditions as well as in response to damage (5,6). SMG-1 has also been shown to affect cell death receptor signaling and to protect cells from extrinsically induced apoptotic cell death (7).

  1. Denning, G. et al. (2001) J Biol Chem 276, 22709-14.
  2. Yamashita, A. et al. (2001) Genes Dev 15, 2215-28.
  3. Brumbaugh, K.M. et al. (2004) Mol Cell 14, 585-98.
  4. Ohnishi, T. et al. (2003) Mol Cell 12, 1187-200.
  5. Li, Z.Y. et al. (2006) Curr Med Chem 13, 1693-705.
  6. Mendell, J.T. et al. (2004) Nat Genet 36, 1073-8.
  7. Oliveira, V. et al. (2008) J Biol Chem 283, 13174-84.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products