Product Pathways - MAPK Signaling
Phospho-SEK1/MKK4 (Thr261) Antibody #9151
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H M R | 46 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-SEK1/MKK4 (Thr261) Antibody detects endogenous levels of SEK1/MKK4 protein only when phosphorylated at threonine 261. This antibody does not cross-react with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH coupled) corresponding to residues around Thr261 of human SEK1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from untreated and UV treated 3T3, C6 and 293 cells, using Phospho-SEK1/MKK4 (Thr261) Antibody.
Background
SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through phosphorylation of serine and threonine residues at positions 257 and 261, respectively, by MEKK. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).
- Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
- Sanchez, I. et al. (1994) Nature 372, 794-798.
- Yan, M. et al. (1994) Nature 372, 798-800.
- Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
- Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.
Application References
- Benhar, M. et al. (2001) Enhanced ROS production in oncogenically transformed cells potentiates c-Jun N-terminal kinase and p38 mitogen-activated protein kinase activation and sensitization to genotoxic stress. Mol. Cell. Biol. 21 (20), 6913-6926. This article references the use of Phospho-SEK1/MKK4 (Thr261) Antibody in the following applications: Western Blotting
- Boppart, M. D. et al. (2000) Marathon running transiently increases c-Jun NH2-terminal kinase and p38 activities in human skeletal muscle. J. Cell. Physiol. 526, 663-669. This article references the use of Phospho-SEK1/MKK4 (Thr261) Antibody in the following applications: Western Blotting
- Wolfman, J. C. et al. (2002) Cellular N-Ras promotes cell survival by downregulation of Jun N-terminal protein kinase and p38. Molec. Cell. Biol. 22, 1589-1606. This article references the use of Phospho-SEK1/MKK4 (Thr261) Antibody in the following applications: Western Blotting
- Bourbon, N. A. et al. (2000) Ceramide directly activates protein kinase C zeta to regulate a stress-activated protein kinase signaling complex. J. Biol. Chem. 275, 35617-35623. This article references the use of Phospho-SEK1/MKK4 (Thr261) Antibody in the following applications: Western Blotting
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