Cell Signaling Technology

Product Pathways - MAPK Signaling

SEK1/MKK4 Antibody #9152

Applications Reactivity MW (kDa) Source
W IP H M R Mk 44 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

SEK1/MKK4 Antibody detects endogenous levels of total SEK1/MKK4 protein. This antibody does not cross-react with MEK1, MEK2 or MKK3.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to residues surrounding the N-terminus of human SEK1/MKK4. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293, NIH/3T3, C6, COS and A431 cells, using SEK1/MKK4 Antibody.

Background

SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through phosphorylation of serine and threonine residues at positions 257 and 261, respectively, by MEKK. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).

  1. Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
  2. Sanchez, I. et al. (1994) Nature 372, 794-798.
  3. Yan, M. et al. (1994) Nature 372, 798-800.
  4. Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
  5. Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.

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