Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

LATS1 Antibody #9153

Applications Reactivity Sensitivity MW (kDa) Source
W H Mk Endogenous 140 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

LATS1 Antibody detects endogenous levels of total LATS1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acids surrounding Ser177 of human LATS1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from K562, MOLT4 and SH-SY5Y cells using LATS1 Antibody.

Background

LATS1 (Large tumor suppressor 1) is putative serine/threonine kinase that belongs to the NDR family (1). It is a tumor suppressor that plays a critical role in maintenance of ploidy. LATS1 localizes to the centrosome and the mitotic spindle and controls G(2)/M transition by negatively regulating CDC2 kinase activity (2,3). It also plays a role in the G1 tetraploidy checkpoint via control of p53 expression (4).LATS1 affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1 (5). LATS1 also binds the phosphorylated form of zyxin, a regulator of actin filament assembly. This interaction promotes localization of zyxin to the mitotic spindle, suggesting a role for actin regulatory proteins during mitosis (6). Decreased expression is associated with breast tumor aggressiveness (7), promoter methylation and loss of heterozygosity. Mutations perturbing LATS1 have been associated with human sarcomas and ovarian sarcomas (8,9). LATS1 knock out mice develop soft-tissue sarcomas, ovarian stromal cell tumors and display a high sensitivity to carcinogenic treatments (10).It has recently been shown that human LATS1 exists in a complex similar to the Drosophila Hippo/Salvador/Lats tumor suppressor network, a complex that regulates proliferation and apoptosis to control growth and shape of the fly. The human complex contains Hippo and Salvador homologs RASSF1A, WW45 and also MST2 and may control mitotic exit (11).

  1. Tao, W. et al. (1999) Nat Genet 21, 177-81.
  2. Yang, X. et al. (2001) Oncogene 20, 6516-23.
  3. Xia, H. et al. (2002) Oncogene 21, 1233-41.
  4. Iida, S. et al. (2004) Oncogene 23, 5266-74.
  5. Yang, X. et al. (2004) Nat Cell Biol 6, 609-17.
  6. Hirota, T. et al. (2000) J Cell Biol 149, 1073-86.
  7. Morinaga, N. et al. (2000) Int J Oncol 17, 1125-9.
  8. Hansen, L.L. et al. (2002) Cancer Genet Cytogenet 139, 1-8.
  9. Hisaoka, M. et al. (2002) Lab Invest 82, 1427-35.
  10. St John, M.A. et al. (1999) Nat Genet 21, 182-6.
  11. Guo, C. et al. (2007) Curr Biol 17, 700-5.

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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