Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody #9156

Applications Reactivity MW (kDa) Source
W H M R Dm (X) (Z) 44 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Dm=D. melanogaster  X=Xenopus  Z=Zebra Fish
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody detects endogenous levels of SEK1/MKK4 phosphorylated at serine 257/threonine 261 . This antibody does not cross-react with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH coupled) corresponding to residues surrounding Ser257/Thr261 of human SEK1/MKK4. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, A431 and PC12 cells, untreated or sorbitol-treated, using Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody (upper) or SEK1/MKK4 Antibody #9152 (lower).

Background

SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through phosphorylation of serine and threonine residues at positions 257 and 261, respectively, by MEKK. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).

  1. Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
  2. Sanchez, I. et al. (1994) Nature 372, 794-798.
  3. Yan, M. et al. (1994) Nature 372, 798-800.
  4. Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
  5. Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.

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