Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Merlin (Ser518) Antibody #9163

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 70 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Merlin (Ser518) Antibody detects endogenous levels of merlin only when phosphorylated at Ser518.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human merlin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from PC3 cells, untreated or treated with λ phosphatase, using Phospho-Merlin (Ser518) Antibody (upper) or Merlin Antibody #9168 (lower).

Background

Neurofibromatosis 2 (NF2) is an autosomal dominant inherited disorder that is characterized by the occurrence of vestibular schwannomas, meningiomas, and other nervous system tumors. Both the familial tumors of NF2 and equivalent sporadic tumors in the general population are caused by inactivation of the NF2 tumor suppressor gene. Merlin (moesin, ezrin, and radixin like protein), the NF2 gene product, has striking similarity to ezrin, radixin, and moesin (ERM). Regulation of merlin (aka schwannomin) and ERM proteins involves intramolecular and intermolecular head-to-tail associations between family members (1). Merlin and ERM proteins act as linkers between the plasma membrane and the cytoskeleton, affecting cell morphology, polarity, and signal transduction (2). Merlin is phosphorylated by the Rac/Cdc42 effector p21-activated kinase (PAK) at Ser518, negatively regulating Rac (3,4).

  1. Ramesh, V. (2004) Nat. Rev. Neurosci. 5, 462-470.
  2. Bretscher, A. et al. (2002) Nat. Rev. Mol. Cell Biol. 3, 586-599.
  3. Xiao, G. H. et al. (2002) J. Biol. Chem. 277, 883-886.
  4. Kissil, J. L. et al. (2003) Mol. Cell 12, 841-849.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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