Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-c-Jun (Ser73) Antibody #9164

Applications Reactivity MW (kDa) Source
W IHC-P IF-IC H M R 48 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-c-Jun (Ser73) Antibody detects endogenous levels of c-Jun only when activated by phosphorylation at Ser73. This antibody also recognizes JunD phosphorylated at Ser100.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues around Ser73 of human c-Jun. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from UV-treated NIH/3T3 cells using Phospho-c-Jun (Ser73) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells treated with anisomycin (25 ug/ml) for the indicated times, using Phospho-c-Jun (Ser73) Antibody #9164. The lower molecular weight band is phospho-JunD (Ser100).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-c-Jun (Ser73) Antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma control (left) or lambda phosphatase-treated (right), using Phospho-c-Jun (Ser73) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-c-Jun (Ser73) Antibody in the presence of control peptide (left) or Phospho-c-Jun (Ser73) Blocking Peptide #1030 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma, using Phospho-c-Jun (Ser73) Antibody.


IHC-FL (floating)

IHC-FL (floating)

Double immunostaining of rat hippocampal CA1 neurons, control and after transient cerebral ischemia, followed by reperfusion, using Phospho-c-Jun (Ser73) Antibody (green) and calbindin (red). Yellow represents overlay of red and green. (Provided by Dr. Bingren Hu, University of Miami School of Medicine, Florida.)

IF-IC

IF-IC

Confocal immunofluorescent images of C6 cells, untreated (left) or anisomycin treated (right), labeled with Phospho-c-Jun (Ser73) Antibody (green) and beta-Tubulin Antibody #2146 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2). Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress-induced signaling pathways. The MAP kinase homologue SAPK/JNK binds to the amino-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun (3,4).

  1. Binetruy, B. et al. (1991) Nature 351, 122-127.
  2. Smeal, T. et al. (1991) Nature 354, 494-496.
  3. Derijard, B. et al. (1994) Cell 76, 1025-1037.
  4. Kyriakis, J.M. et al. (1994) Nature 369, 156-160.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Product Pathways

Drug Discovery Tools

Featured Technologies

Protein Classes