Product Pathways - MAPK Signaling

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c-Jun (60A8) Rabbit mAb #9165

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P IHC-F IF-IC	H M R Mk	Endogenous	43, 48	Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

9165:

IHC / Frozen, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting

Specificity / Sensitivity

c-Jun (60A8) Rabbit mAb detects endogenous levels of total c-Jun protein, regardless of phosphorylation state.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST-c-Jun protein corresponding to the amino-terminal sequence of human c-Jun.

Background

c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).

1. Jochum, W. et al. (2001) Oncogene 20, 2401-12.
2. Davis, R.J. (2000) Cell 103, 239-52.
3. Hilberg, F. et al. (1993) Nature 365, 179-81.
4. Raivich, G. et al. (2004) Neuron 43, 57-67.
5. Behrens, A. et al. (2002) EMBO J 21, 1782-90.
6. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.
7. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
8. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.
9. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.
10. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.
11. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.
12. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.

Application References

• Tokunaga, F. et al. (2009) Nat Cell Biol 11, 123-32. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 9260 PhosphoPlus® c-Jun (Ser63) and c-Jun (Ser73) Antibody Kit
• 9261 Phospho-c-Jun (Ser63) II Antibody
• 9263 c-Jun Control Cell Extracts
• 9164 Phospho-c-Jun (Ser73) Antibody
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7727 Biotinylated Protein Ladder Detection Pack
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8112 SignalStain^® Antibody Diluent

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.