Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

LAT Antibody #9166

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P F H M (R) Endogenous 36, 38 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LAT Antibody detects endogenous levels of total LAT protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the central region of human LAT. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with lambda phosphatase, using LAT Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Jurkat cells, using LAT Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil, showing membrane localization, using LAT Antibody.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, using LAT Antibody (blue) compared to a nonspecific negative control antibody (red).

Background

LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr191 enables the binding of Grb2, Gads/SLP-76, PLCγ1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4).

  1. Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-6283.
  2. Zhang, W. et al. (1998) Cell 92, 83-92.
  3. Paz, P. E. et al. (2001) Biochem. J. 356, 461-471.
  4. Zhang, W. et al. (2000) J. Biol. Chem. 275, 23355-23361.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products