Product Pathways - Jak/Stat Pathway
PhosphoPlus® Stat1 (Tyr701) Antibody Kit #9170
|9170S||1 Kit (10 western blots)||---||In Stock||---|
|9170||carrier free and custom formulation / quantity||email request|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||Mol. Wt.||Isotype|
|Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb #7649||100 µl||W, IP, IF-IC, F, ChIP||H, M, R||Mk||84, 91 kDa||Rabbit IgG|
|Stat1 Antibody #9172||100 µl||W, IP, ChIP||H, M, R, Mk||B, Dg||84, 91 kDa||Rabbit|
|Stat1 Control Cell Extracts #9173||100 µl||Human|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|Anti-biotin, HRP-linked Antibody #7075||100 µl||Goat|
|Biotinylated Protein Ladder Detection Pack #7727||100 µl|
|20X LumiGLO® Reagent and 20X Peroxide #7003||5 ml each|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, ChIP=Chromatin IP
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey
Specificity / Sensitivity
Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb recognizes endogenous levels of Stat1 protein only when phosphorylated at Tyr701. Stat1 antibody detects endogenous levels of total Stat1 α (91kDa) and Stat1 β (84kDa) protein.
Source / Purification
Phospho-Stat1 (Tyr701) (D4A7) monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr701 of human Stat1 protein. Stat1 polyclonal antibody is produced using a synthetic peptide corresponding to a sequence of human Stat1 (Stat1 antibody). Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
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* Product-specific protocol.
For Research Use Only. Not For Use In Diagnostic Procedures.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
LumiGLO® is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.