Product Pathways - Jak/Stat Pathway

Phospho-Stat1 (Tyr701) Antibody #9171

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Applications	Reactivity	MW (kDa)	Source
W IP IF-IC F	H M R (B)	84, 91	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Stat1 (Tyr701) Antibody detects endogenous levels of Stat1 only when phosphorylated at tyrosine 701. The antibody detects phosphorylated tyrosine 701 of p91 Stat1 and also the p84 splice variant. It does not cross-react with the corresponding phospho-tyrosines of other Stat proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr701 of human Stat1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

1. Heim, M.H. (1999) J. Recept. Signal. Transduct. Res. 19, 75-120.
2. Durbin, J.E. et al. (1996) Cell 84, 443-450.
3. Meraz, M.A. et al. (1996) Cell 84, 431-442.
4. Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222-227.
5. Frank, D.A. (1999) Mol. Med. 5, 432-456.
6. Wen, Z. et al. (1995) Cell 82, 241-250.

Application References

• Begitt, A. et al. (2000) Nucleocytoplasmic translocation of Stat1 is regulated by a leucine-rich export signal in the coiled-coil domain. Proc. Natl. Acad. Sci. USA 97, 10418-10423. This article references the use of Phospho-Stat1 (Tyr701) Antibody in the following applications: Western Blotting
• Gamero, A.M. and Larner, A.C. (2000) Signaling via the T cell antigen receptor induces phosphorylation of Stat1 on serine 727. J. Biol. Chem. 275, 16574-16578. This article references the use of Phospho-Stat1 (Tyr701) Antibody in the following applications: Western Blotting
• McBride, K. M. et al. (2000) Nuclear export signal located within the DNA-binding domain of the STAT1 transcription factor. EMBO J. 19, 6196-6206. This article references the use of Phospho-Stat1 (Tyr701) Antibody in the following applications: Western Blotting
• Spagnoli, A. et al. (2002) Identification of STAT-1 as a molecular target of IGFBP-3 in the process of chondrogenesis. J. Biol. Chem. 277, 18860-18867. This article references the use of Phospho-Stat1 (Tyr701) Antibody in the following applications: IC-ABC Western Blotting
• Hirata, A. et al. (2002) ZD1839 (Iressa) induces antiangiogenic effects through inhibition of epidermal growth factor receptor tyrosine kinase. Cancer Res. 62, 2554-2560. This article references the use of Phospho-Stat1 (Tyr701) Antibody in the following applications: Western Blotting
• Hayashi, T. et al. (2002) Regulation of receptor activator of NF-kappaB ligand-induced osteoclastogenesis by endogenous interferon-beta (INF-beta) and suppressors of cytokine signaling (SOCS). J. Biol. Chem. 277, 27880-27886. This article references the use of Phospho-Stat1 (Tyr701) Antibody in the following applications: Western Blotting
• Mowen, K. A. et al. (2001) Arginine methylation of STAT1 modulates IFN alpha/beta-induced transcription. Cell 104, 731-741. This article references the use of Phospho-Stat1 (Tyr701) Antibody in the following applications: Western Blotting
• Teague, T. K. et al. (2000) Activation-induced inhibition of interleukin 6-mediated T cell survival and signal transducer and activator of transcription 1 signaling. J. Exp. Med. 191, 915-926. This article references the use of Phospho-Stat1 (Tyr701) Antibody in the following applications: Western Blotting

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