Product Pathways - Jak/Stat Pathway
Stat1 Control Cell Extracts #9173
|9173S||100 µl (10 western blots)||---||In Stock||---|
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Nonphosphorylated Stat1 Control Cell Extracts: Total cell extracts from HeLa cells prepared without treatment serve as a negative control. Supplied in SDS Sample Buffer.
Phosphorylated Stat1 Control Cell Extracts: Total cell extracts from HeLa cells prepared with 100 ng/ml interferon-alpha 5 minute treatment serve as a positive control. Supplied in SDS Sample Buffer.
As controls, we recommend using 10 µl of phosphorylated and nonphosphorylated Stat1 control cell extracts
These lysates are useful for Phospho-Stat1 (Tyr701) Antibody #9171. However, they are not useful for Phospho-Stat1 (Ser727) Antibody #9177.
The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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