Product Pathways - Jak/Stat Pathway
Stat1 Control Cell Extracts #9173
Nonphosphorylated Stat1 Control Cell Extracts: Total cell extracts from HeLa cells prepared without treatment serve as a negative control. Supplied in SDS Sample Buffer.Phosphorylated Stat1 Control Cell Extracts: Total cell extracts from HeLa cells prepared with 100 ng/ml interferon-alpha 5 minute treatment serve as a positive control. Supplied in SDS Sample Buffer.
As controls, we recommend using 10 µl of phosphorylated and nonphosphorylated Stat1 control cell extractsThese lysates are useful for Phospho-Stat1 (Tyr701) Antibody #9171. However, they are not useful for Phospho-Stat1 (Ser727) Antibody #9177.
The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
- Heim, M.H. (1999) J. Recept. Signal. Transduct. Res. 19, 75-120.
- Durbin, J.E. et al. (1996) Cell 84, 443-450.
- Meraz, M.A. et al. (1996) Cell 84, 431-442.
- Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222-227.
- Frank, D.A. (1999) Mol. Med. 5, 432-456.
- Wen, Z. et al. (1995) Cell 82, 241-250.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 9170 PhosphoPlus® Stat1 (Tyr701) Antibody Kit
- 9171 Phospho-Stat1 (Tyr701) Antibody
- 9172 Stat1 Antibody
- 9914 Phospho-Stat Antibody Sampler Kit
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9175 Stat1 (42H3) Rabbit mAb
- 9939 Stat Antibody Sampler Kit
For Research Use Only. Not For Use In Diagnostic Procedures.