Product Pathways - Jak/Stat Pathway
Stat1 (42H3) Rabbit mAb (Human Specific) #9175
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P | H Mk | Endogenous | 84, 91 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Stat1 (42H3) Rabbit mAb detects endogenous levels of total Stat1 protein independent of phosphorylation.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the sequence of human Stat1.
Western Blotting
Western blot analysis of extracts from HeLa and COS cells, using Stat1 (42H3) Rabbit mAb (Human Specific).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human MALToma, showing cytoplasmic and nuclear staining, using Stat1 (42H3) Rabbit mAb (Human Specific).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using Stat1 (42H3) Rabbit mAb (Human Specific).
IHC-P/IF (paraffin/IF)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Stat1 (42H3) Rabbit mAb (Human Specific) in the presence of control peptide (left) or Stat1 blocking peptide (9175 specific) #1079 (right).
Background
The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
- Heim, M.H. (1999) J. Recept. Signal. Transduct. Res. 19, 75-120.
- Durbin, J.E. et al. (1996) Cell 84, 443-450.
- Meraz, M.A. et al. (1996) Cell 84, 431-442.
- Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222-227.
- Frank, D.A. (1999) Mol. Med. 5, 432-456.
- Wen, Z. et al. (1995) Cell 82, 241-250.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7120 PathScan® Multiplex Western Cocktail III: Phospho-Stat1, Phospho-SAPK/JNK, Phospho-S6 Ribosomal Protein and Phospho-HSP27 Detection Kit
- 9170 PhosphoPlus® Stat1 (Tyr701) Antibody Kit
- 6331 SignalSilence® Stat1 siRNA
- 6330 SignalSilence® Stat1 siRNA Kit
- 9171 Phospho-Stat1 (Tyr701) Antibody
- 9172 Stat1 Antibody
- 9173 Stat1 Control Cell Extracts
- 9176 Stat1 (9H2) Mouse mAb
- 9177 Phospho-Stat1 (Ser727) Antibody
- 8105 SignalSlide™ Phospho-Stat1/3/5 IHC Controls
- 1079 Stat1 Blocking Peptide (9175 Specific)
- 8112 SignalStain® Antibody Diluent
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
