Product Pathways - Jak/Stat Pathway
Stat1 (42H3) Rabbit mAb #9175
|9175S||100 µl (10 western blots)||---||In Stock||---|
|9175||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||84, 91||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
Stat1 (42H3) Rabbit mAb detects endogenous levels of total Stat1 protein independent of phosphorylation.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Stat1.
Western blot analysis of extracts from HeLa and COS cells, using Stat1 (42H3) Rabbit mAb (Human Specific).
Immunohistochemical analysis of paraffin-embedded human MALToma, showing cytoplasmic and nuclear staining, using Stat1 (42H3) Rabbit mAb (Human Specific).
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using Stat1 (42H3) Rabbit mAb (Human Specific).
The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.