Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Mer (348E6) Mouse mAb #9178

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 210 Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Mer (348E6) Mouse mAb detects endogenous levels of Mer proteins. It does not cross-react with other receptor tyrosine kinase family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human Mer extracellular domain produced in Sf21 cells.

Western Blotting

Western Blotting

Western blot analysis of U937 cell lysate using Mer (348E6) Mouse mAb.

Background

Mer tyrosine kinase belongs to a receptor tyrosine kinase family with Axl and Tyro3. This family is characterized by a common NCAM (neural adhesion molecule)-related extracellular domain and a common ligand, GAS6 (growth arrest-specific protein 6). Mer protein has an apparent molecular weight of 170-210 kDa due to different glycosylation patterns generated in different cell types. Mer can be activated by dimerization and autophosphorylation through ligand binding or homophilic cell-cell interaction mediated by its NCAM-like motif (1). The downstream signaling components of activated Mer include PI3 kinase, PLCγ, and MAP kinase (2). Family members are prone to transcriptional regulation and carry out diverse functions including the regulation of cell adhesion, migration, phagocytosis, and survival (3). Mer regulates macrophage activation, promotes apoptotic cell engulfment, and supports platelet aggregation and clot stability in vivo (4). Investigators ahve found that overexpression of Mer may play a cooperative role in leukemogenesis and may be an effective target for biologically based leukemia/lymphoma therapy (5).

  1. Ling, L. et al. (1996) J. Biol. Chem. 271, 18355-18362.
  2. Ling, L. and Kung, H.J. (1995) Mol. Cell Biol. 15, 6582-6592.
  3. Hafizi, S. and Dahlbäck, B. (2006) Cytokine Growth Factor Rev. 17, 295-304.
  4. Sather, S. et al. (2007) Blood 109, 1026-1033.
  5. Keating, A.K. et al. (2006) Oncogene 25, 6092-6100.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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