Product Pathways - MAPK Signaling
PhosphoPlus® Elk-1 (Ser383) Antibody Kit #9180
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|---|---|
| Phospho-Elk-1 (Ser383) Antibody # 9181 | 100 microliters | W | (H) (M) (R) (Mk) (Z) (Dg) | 47-60 | Rabbit |
| Elk-1 Antibody # 9182 | 100 microliters | W | H M R Z | 47-60 | Rabbit |
| Elk-1 Control Proteins # 9183 | 60 microliters | 40 kDa nonphosphorylated, 50 kDa phosphorylated | E. Coli | ||
| Elk-1 Control Proteins # 9183 | 60 microliters | 40 kDa nonphosphorylated, 50 kDa phosphorylated | E. Coli | ||
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 50 microliters | Goat | |||
| Anti-biotin, HRP-linked Antibody # 7075 | 100 microliters | Goat | |||
| Biotinylated Protein Ladder Detection Pack # 7727 | 100 microliters | ||||
| 20X LumiGLO® Reagent and 20X Peroxide # 7003 | 5 milliliters | ||||
| 20X LumiGLO® Reagent and 20X Peroxide # 7003 | 5 milliliters |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Z=Zebra Fish
Dg=Dog
Specificity / Sensitivity
Western Blotting
Western analysis of Elk-1 fusion protein expressed from E. coli with or without phosphorylation with purified Erk2 enzyme using Phospho-Elk-1 (Ser383) (A) or Elk-1 Antibodies (B).
Source / Purification
Background
The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).
- Marais, R. et al. (1993) Cell 73, 381-393.
- Kortenjann, M. et al. (1994) Mol. Cell. Biol. 14, 4815-4824.
- Hill, C.S. and Treisman, R. (1995) Cell 80, 199-211.
- Cavigelli, M. et al. (1995) EMBO J. 14, 5957-5964.
- Whitmarsh, A.J. et al. (1995) Science 269, 403-407.
Application References
- Aplin, A. E. et al. (2001) Integrin-mediated adhesion regulates ERK nuclear translocation and phosphorylation of Elk-1. J. Biol. Chem. 153, 273-282. This article references the use of PhosphoPlus® Elk-1 (Ser383) Antibody Kit in the following applications: IC-ABC Western Blotting
- Hu, Y. et al. (2000) E-selectin-dependent signaling via the mitogen-activated protein kinase pathway in vascular endothelial cells. J. Immunol. 165, 2142-2418. This article references the use of PhosphoPlus® Elk-1 (Ser383) Antibody Kit in the following applications: Western Blotting
- Wang, D. and Richmond, A. (2001) Nuclear factor-kappa B activation by the CXC chemokine melanoma growth-stimulatory activity/growth-regulated protein involves the MEKK1/p38 mitogen-activated protein kinase pathway. J. Biol. Chem. 276, 3650-3659. This article references the use of PhosphoPlus® Elk-1 (Ser383) Antibody Kit in the following applications: Western Blotting
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Companion Products
- 9100 PhosphoPlus® p44/42 MAP Kinase (Thr202/Tyr204) Antibody Kit
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 9181 Phospho-Elk-1 (Ser383) Antibody
- 9186 Phospho-Elk-1 (Ser383) (2B1) Mouse mAb
- 9182 Elk-1 Antibody
- 9183 Elk-1 Control Proteins
- 9184 Elk-1 Fusion Protein
