Product Pathways - MAPK Signaling
Phospho-Elk-1 (Ser383) Antibody #9181
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | (H) (M) (R) (Mk) (Z) (Dg) | 47-60 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Z=Zebra Fish
Dg=Dog
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Elk-1 (Ser383) Antibody detects less than 5 ng of phosphorylated Elk-1, and will not react with up to 1 µg of nonphosphorylated EIk-1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH coupled) corresponding to residues around Ser383 of human Elk-1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of Elk-1 fusion protein expressed from E. coli with or without phosphorylation by purified Erk2 enzyme, using Phospho-Elk-1 (Ser383) Antibody (upper) or control Elk-1 Antibody #9182 (lower).
Background
The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).
- Marais, R. et al. (1993) Cell 73, 381-393.
- Kortenjann, M. et al. (1994) Mol. Cell. Biol. 14, 4815-4824.
- Hill, C.S. and Treisman, R. (1995) Cell 80, 199-211.
- Cavigelli, M. et al. (1995) EMBO J. 14, 5957-5964.
- Whitmarsh, A.J. et al. (1995) Science 269, 403-407.
Application References
- Aplin, A. E. et al. (2001) Integrin-mediated adhesion Regulates ERK Nuclear Translocation and Phosphorylation of Elk-1. J. Cell Biol. 153, 273-282. This article references the use of Phospho-Elk-1 (Ser383) Antibody in the following applications: IC-IF Western Blotting
- Hu, Y. et al. (2000) E-selectin-dependent signaling via the mitogen-activated protein kinase pathway in vascular endothelial cells. J. Immunol. 165, 2142-2148. This article references the use of Phospho-Elk-1 (Ser383) Antibody in the following applications: Western Blotting
- Stewart, S. and Guan, K.L. (2000) The dominant negative Ras mutant, N17 Ras, can inhibit signaling independently of blocking Ras activation. J. Biol. Chem. 275, 8854-8862. This article references the use of Phospho-Elk-1 (Ser383) Antibody in the following applications: Western Blotting
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Companion Products
- 9182 Elk-1 Antibody
- 9183 Elk-1 Control Proteins
- 9184 Elk-1 Fusion Protein
- 9186 Phospho-Elk-1 (Ser383) (2B1) Mouse mAb
- 9800 p44/42 MAP Kinase Assay Kit (Nonradioactive)
- 9911 Phospho-Erk1/2 Pathway Sampler Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
