Product Pathways - MAPK Signaling
Elk-1 Control Proteins #9183
|9183S||150 µl (10 western blots)||---||In Stock||---|
|9183||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
40 kDa nonphosphorylated, 50 kDa phosphorylated
Nonphosphorylated Elk1 fusion protein: Bacterially expressed Elk1 fusion protein serves as a negative control. Supplied in SDS Sample Buffer. Store at -20ºC.
Phosphorylated Elk1 fusion protein: Elk1 phosphorylated by the Erk2 enzyme serves as a positive control. Supplied in SDS Sample Buffer. Store at -20ºC.
Directions For Use
As controls, we recommend using 15 µl of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.
The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).
Western Blots: For controls, we recommend using 15 µl of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.