Product Pathways - MAPK Signaling
Phospho-Elk-1 (Ser383) (2B1) Mouse mAb #9186
PhosphoSitePlus® protein, site, and accession data: ELK1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | (H) (M) (R) | Recombinant | 47 | Mouse IgG1 |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9186:
- Western Blotting
Specificity / Sensitivity
Phospho-Elk-1 (Ser383) (2B1) Mouse mAb detects less than 5 ng of phosphorylated Elk-1, and will not react with up to 1 µg of nonphosphorylated Elk-1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser383 of human Elk-1.
Western Blotting
Western blot analysis of Elk-1 fusion protein expressed from E. coli with or without phosphorylation with purified Erk2 enzyme, using Phospho-Elk-1 (Ser383) (2B1) Mouse mAb (upper) or Elk-1 Antibody #9182 (lower).
Background
The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).
- Marais, R. et al. (1993) Cell 73, 381-393.
- Kortenjann, M. et al. (1994) Mol. Cell. Biol. 14, 4815-4824.
- Hill, C.S. and Treisman, R. (1995) Cell 80, 199-211.
- Cavigelli, M. et al. (1995) EMBO J. 14, 5957-5964.
- Whitmarsh, A.J. et al. (1995) Science 269, 403-407.
Application References
- Plotnikov, A. et al. (2011) Mol Cell Biol 31, 3515-30. Applications: Western Blotting
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Companion Products
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
