Product Pathways - Neuroscience

Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate) #9187

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Applications	Reactivity	Source	Isotype
IF-F IF-IC F	H M	Rabbit	IgG

Applications Key:  IF-F=Immunofluorescence (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-CREB (Ser133) (87G3) (Alexa Fluor® 488 Conjugate) Rabbit mAb detects endogenous levels of CREB only when phosphorylated at serine 133. The antibody also detects the phosphorylated form of the CREB-related protein, ATF-1.

Source / Purification

Monoclonal antibody is produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser133 of human CREB. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-5.

Description

Cell Signaling Technology Antibody conjugated to Alexa Fluor ® 488 fluorescent dye and tested in-house for direct Flow Cytometric analysis of human and mouse cells. The unconjugated antibody, #9198 reacts with Phospho-CREB (Ser133) from human, mouse and rat. CST expects that Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize Phospho-CREB in these species.

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (7-9).

1. Lonze, B.E. et al. (2002) Neuron 34, 371-385.
2. Lee, M.M. et al. (1999) J. Neurosci. Res. 55, 702-712.
3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
4. Dash, P.K. et al. (1990) Nature 345, 718-721.
5. Yin, J.C. et al. (1994) Cell 79, 49-58.
6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc. Nat. Acad. Sci. USA 94, 2693-2698.
7. Xing, J. et al. (1998) Mol. Cell. Biol. 18, 1946-1955.
8. Ribar, T.J. et al. (2000) J. Neurosci. 20, RC107.
9. Tan, Y. et al. (1996) EMBO J. 15, 4629-4642.

Application References

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Companion Products

• 4340 Rabbit IgG Isotype Control (Alexa Fluor^® 488 Conjugate)
• 1090 Phospho-CREB (Ser133) Blocking Peptide
• 9104 CREB (86B10) Mouse mAb
• 9190 PhosphoPlus® CREB (Ser133) Antibody Kit
• 9191 Phospho-CREB (Ser133) Antibody
• 9193 CREB Control Cell Extracts
• 9196 Phospho-CREB (Ser133) (1B6) Mouse mAb
• 9197 CREB (48H2) Rabbit mAb
• 9198 Phospho-CREB (Ser133) (87G3) Rabbit mAb

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.