Cell Signaling Technology

Product Pathways - Metabolism

ACSL1 (D2H5) Rabbit mAb #9189

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 78 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ACSL1 (D2H5) Rabbit mAb recognizes endogenous levels of total ACSL1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala257 of human ACSL1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ACSL1 (D2H5) Rabbit mAb.

Background

Mammalian long-chain acyl-CoA synthetase (ACSL) catalyzes the ligation of the fatty acid to CoA to form fatty acyl-CoA in a two-step reaction (1). Five isoforms of ACSL have been identified (1). These isoforms have different substrate preferences and subcellular localizations (1). Overexpression of ACSL1 results in changes to fatty acid metabolism in rat primary hepatocytes (2).

  1. Mashek, D.G. et al. (2004) J Lipid Res 45, 1958-61.
  2. Li, L.O. et al. (2006) J Biol Chem 281, 37246-55.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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