Product Pathways - Neuroscience
PhosphoPlus® CREB (Ser133) Antibody Kit #9190
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|---|---|
| CREB (48H2) Rabbit mAb # 9197 | 100 microliters | W IP IHC-P IHC-F IF-F IF-IC F | H M R Mk Dr | 43 | Rabbit |
| Phospho-CREB (Ser133) (87G3) Rabbit mAb # 9198 | 100 microliters | W IHC-P IHC-F IF-F IF-IC F | H M R | 43 | Rabbit |
| CREB Control Cell Extracts # 9193 | 40 microliters | ||||
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 50 microliters | Goat | |||
| Anti-biotin, HRP-linked Antibody # 7075 | 100 microliters | Goat | |||
| 20X LumiGLO® Reagent and 20X Peroxide # 7003 | 5 milliliters | ||||
| Biotinylated Protein Ladder Detection Pack # 7727 | 100 microliters |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-F=Immunofluorescence (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Dr=Drosophila
Specificity / Sensitivity
Phospho-CREB (Ser133) (87G3) Rabbit mAb detects endogenous levels of CREB only when phosphorylated at Ser133. This antibody also detects the phosphorylated form of CREB-related protein ATF-1. CREB (48H2) Rabbit mAb detects endogenous levels of total CREB protein.
Western Blotting
Western blot analysis of extracts from SK-N-MC cells, untreated or forskolin- and FGF-treated, using Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 (upper) or CREB (48H2) Rabbit mAb #9197 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CREB (48H2) Rabbit mAb #9197.
IHC-F (frozen)
Immunohistochemical analysis of frozen H1650 xenograft, showing nuclear localization using CREB (48H2) Rabbit mAb #9197.
Flow Cytometry
Flow cytometric analysis of SK-N-MC cells, using CREB (48H2) Rabbit mAb #9197 (blue) compared to a nonspecific negative control antibody (red).
Flow Cytometry
Flow cytometric analysis of SK-N-MC cells, untreated (blue) or IBMX- and forskolin-treated (green), using Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 compared to a nonspecific negative control antibody (red).
IF-F
Confocal immunofluorescent analysis of mouse cerebellum labeled with CREB (48H2) Rabbit mAb #9197 (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor =DRAQ5® (fluorescent DNA dye).
IF-F
Conofocal immunofluorescent images of rat dentate gyrus, either sham-operated (left) or 15 min ischemia followed by 30 min (center) and 4 h (right) reperfusion, labeled with Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 (red), Neurofilament-L (DA2) Mouse mAb #2835 (blue) and Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854.
Source / Purification
Rabbit monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser133 of human CREB (Phospho-CREB (Ser133) (87G3) Rabbit mAb), or with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxy-terminus of human CREB (CREB (48H2) Rabbit mAb).
Background
CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (7-9).
- Lonze, B.E. et al. (2002) Neuron 34, 371-385.
- Lee, M.M. et al. (1999) J. Neurosci. Res. 55, 702-712.
- Redmond, L. et al. (2002) Neuron 34, 999-1010.
- Dash, P.K. et al. (1990) Nature 345, 718-721.
- Yin, J.C. et al. (1994) Cell 79, 49-58.
- Guzowski, J.F. and McGaugh, J.L. (1997) Proc. Nat. Acad. Sci. USA 94, 2693-2698.
- Xing, J. et al. (1998) Mol. Cell. Biol. 18, 1946-1955.
- Ribar, T.J. et al. (2000) J. Neurosci. 20, RC107.
- Tan, Y. et al. (1996) EMBO J. 15, 4629-4642.
Application References
- Constantinescu, A. et al. (2002) cAMP-dependent protein kinase types I and II differentially regulate cAMP response element-mediated gene expression: implications for neuronal responses to ethanol. J. Biol. Chem. 277, 18810-18816. This article references the use of PhosphoPlus® CREB (Ser133) Antibody Kit in the following applications: IC-ABC
- Liu, X. and Green, C.B. (2002) Circadian regulation of nocturnin transcription by phosphorylated CREB in Xenopus retinal photoreceptor cells. Mol. Cell. Biol. 22, 7501-7511. This article references the use of PhosphoPlus® CREB (Ser133) Antibody Kit in the following applications: IHC-FL (floating/frozen)
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Companion Products
- 9104 CREB (86B10) Mouse mAb
- 9191 Phospho-CREB (Ser133) Antibody
- 9193 CREB Control Cell Extracts
- 9196 Phospho-CREB (Ser133) (1B6) Mouse mAb
- 9197 CREB (48H2) Rabbit mAb
- 9198 Phospho-CREB (Ser133) (87G3) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 9187 Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate)
Rabbit Monoclonals Produced UsingTechnology from Epitomics, Inc. UnderU.S. patent No. 5,675,063.
