Product Pathways - Neuroscience
PhosphoPlus® CREB (Ser133) Antibody Kit #9190
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| CREB (48H2) Rabbit mAb # 9197 | 100 microliters | W IP IHC-P IHC-F IF-F IF-IC F ChIP | H M R Mk Dm | 43 | Rabbit IgG |
| Phospho-CREB (Ser133) (87G3) Rabbit mAb # 9198 | 100 microliters | W IHC-P IHC-F IF-F IF-IC F ChIP | H M R | 43 | Rabbit IgG |
| CREB Control Cell Extracts # 9193 | 40 microliters | ||||
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 50 microliters | Goat | |||
| Anti-biotin, HRP-linked Antibody # 7075 | 100 microliters | Goat | |||
| 20X LumiGLO® Reagent and 20X Peroxide # 7003 | 5 milliliters | ||||
| Biotinylated Protein Ladder Detection Pack # 7727 | 100 microliters |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-F=Immunofluorescence (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
ChIP=Chromatin IP
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Dm=D. melanogaster
Specificity / Sensitivity
Phospho-CREB (Ser133) (87G3) Rabbit mAb detects endogenous levels of CREB only when phosphorylated at Ser133. This antibody also detects the phosphorylated form of CREB-related protein ATF-1. CREB (48H2) Rabbit mAb detects endogenous levels of total CREB protein.
Western Blotting
Western blot analysis of extracts from SK-N-MC cells, untreated or forskolin- and FGF-treated, using Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 (upper) or CREB (48H2) Rabbit mAb #9197 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CREB (48H2) Rabbit mAb #9197.
IHC-F (frozen)
Immunohistochemical analysis of frozen H1650 xenograft, showing nuclear localization using CREB (48H2) Rabbit mAb #9197.
Flow Cytometry
Flow cytometric analysis of SK-N-MC cells, using CREB (48H2) Rabbit mAb #9197 (blue) compared to a nonspecific negative control antibody (red).
Flow Cytometry
Flow cytometric analysis of SK-N-MC cells, untreated (blue) or IBMX- and forskolin-treated (green), using Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 compared to a nonspecific negative control antibody (red).
IF-F
Confocal immunofluorescent analysis of mouse cerebellum labeled with CREB (48H2) Rabbit mAb #9197 (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor =DRAQ5® (fluorescent DNA dye).
IF-F
Conofocal immunofluorescent images of rat dentate gyrus, either sham-operated (left) or 15 min ischemia followed by 30 min (center) and 4 h (right) reperfusion, labeled with Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 (red), Neurofilament-L (DA2) Mouse mAb #2835 (blue) and Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854.
Description
The PhosphoPlus CREB (Ser133) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of CREB at Ser133.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB (Phospho-CREB (Ser133) (87G3) Rabbit mAb), or with a synthetic peptide corresponding to residues near the carboxy-terminus of human CREB (CREB (48H2) Rabbit mAb).
Background
CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (7-9).
- Lonze, B.E. et al. (2002) Neuron 34, 371-85.
- Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
- Redmond, L. et al. (2002) Neuron 34, 999-1010.
- Dash, P.K. et al. (1990) Nature 345, 718-21.
- Yin, J.C. et al. (1994) Cell 79, 49-58.
- Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
- Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
- Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
- Tan, Y. et al. (1996) EMBO J 15, 4629-42.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Protocols
- 9197:
- ChIP Agarose, ChIP Magnetic, Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
- 9198:
- ChIP Agarose, ChIP Magnetic, Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence, Western Blotting
Companion Products
- 9104 CREB (86B10) Mouse mAb
- 9191 Phospho-CREB (Ser133) Antibody
- 9193 CREB Control Cell Extracts
- 9196 Phospho-CREB (Ser133) (1B6) Mouse mAb
- 9197 CREB (48H2) Rabbit mAb
- 9198 Phospho-CREB (Ser133) (87G3) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 9187 Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate)
Rabbit Monoclonals Produced UsingTechnology from Epitomics, Inc. UnderU.S. patent No. 5,675,063.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
