Product Pathways - Neuroscience
PhosphoPlus® CREB (Ser133) Antibody Kit #9190
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|CREB (48H2) Rabbit mAb #9197||100 µl||W IP IHC-P IHC-F IF-F IF-IC F ChIP||H M R Mk Dm||43||Rabbit IgG|
|Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198||100 µl||W IHC-P IHC-F IF-F IF-IC F ChIP||H M R (Z)||43||Rabbit IgG|
|CREB Control Cell Extracts #9193||100 µl|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|Anti-biotin, HRP-linked Antibody #7075||100 µl||Goat|
|20X LumiGLO® Reagent and 20X Peroxide #7003||5 ml each|
|Biotinylated Protein Ladder Detection Pack #7727||100 µl|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster Z=Zebrafish
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-CREB (Ser133) (87G3) Rabbit mAb detects endogenous levels of CREB only when phosphorylated at Ser133. This antibody also detects the phosphorylated form of CREB-related protein ATF-1. CREB (48H2) Rabbit mAb detects endogenous levels of total CREB protein.
Western blot analysis of extracts from SK-N-MC cells, untreated or forskolin- and FGF-treated, using Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 (upper) or CREB (48H2) Rabbit mAb #9197 (lower).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CREB (48H2) Rabbit mAb #9197.
Immunohistochemical analysis of frozen H1650 xenograft, showing nuclear localization using CREB (48H2) Rabbit mAb #9197.
Flow cytometric analysis of SK-N-MC cells, using CREB (48H2) Rabbit mAb #9197 (blue) compared to a nonspecific negative control antibody (red).
Flow cytometric analysis of SK-N-MC cells, untreated (blue) or IBMX- and forskolin-treated (green), using Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 compared to a nonspecific negative control antibody (red).
Confocal immunofluorescent analysis of mouse cerebellum labeled with CREB (48H2) Rabbit mAb #9197 (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor =DRAQ5® (fluorescent DNA dye).
Conofocal immunofluorescent images of rat dentate gyrus, either sham-operated (left) or 15 min ischemia followed by 30 min (center) and 4 h (right) reperfusion, labeled with Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 (red), Neurofilament-L (DA2) Mouse mAb #2835 (blue) and Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854.
The PhosphoPlus CREB (Ser133) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of CREB at Ser133.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB (Phospho-CREB (Ser133) (87G3) Rabbit mAb), or with a synthetic peptide corresponding to residues near the carboxy-terminus of human CREB (CREB (48H2) Rabbit mAb).
CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).
- Lonze, B.E. et al. (2002) Neuron 34, 371-85.
- Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
- Redmond, L. et al. (2002) Neuron 34, 999-1010.
- Dash, P.K. et al. (1990) Nature 345, 718-21.
- Yin, J.C. et al. (1994) Cell 79, 49-58.
- Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
- Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
- Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
- Tan, Y. et al. (1996) EMBO J 15, 4629-42.
- Constantinescu, A. et al. (2002) J Biol Chem 277, 18810-6. Applications: IC-ABC
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- 9104 CREB (86B10) Mouse mAb
- 9191 Phospho-CREB (Ser133) Antibody
- 9193 CREB Control Cell Extracts
- 9196 Phospho-CREB (Ser133) (1B6) Mouse mAb
- 9197 CREB (48H2) Rabbit mAb
- 9198 Phospho-CREB (Ser133) (87G3) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 9187 Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate)
Rabbit Monoclonals Produced UsingTechnology from Epitomics, Inc. UnderU.S. patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.