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Phospho-CREB (Ser133) Antibody #9191

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H M R	Endogenous	43	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

9191:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Phospho-CREB (Ser133) Antibody detects endogenous levels of CREB only when phosphorylated at serine 133. This antibody also detects the phosphorylated form of the CREB-related protein ATF-1, and has been shown to react weakly with overexpressed levels of pyruvate dehydrogenase.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca²⁺, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
4. Dash, P.K. et al. (1990) Nature 345, 718-21.
5. Yin, J.C. et al. (1994) Cell 79, 49-58.
6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.

Application References

• Grewal, S. S. et al. (2000) Calcium and cAMP signals differentially regulate cAMP-responsive element-binding protein function via a Rap1-extracellular signal-regulated kinase pathway. J. Biol. Chem. 275, 34433-34441. Applications: ICC-IF Western Blot
• Reusch, J. E. et al. (2000) CREB activation induces adipogenesis in 3T3-L1 cells. Mol. Cell. Biol. 20, 1008-1020. Applications: Western Blot
• Ho, N. et al. (2000) Impaired synaptic plasticity and cAMP response element-binding protein activation in Ca2+/calmodulin-dependent protein kinase type IV/Gr-deficient mice. J. Neurosci. 20, 6459-6472. Applications: IHC-F (floating) Western Blot
• Huang, H. et al. (2001) PTEN induces chemosensitivity in PTEN-mutated prostate cancer cells by suppression of Bcl-2 expression. J. Biol. Chem. 276, 38830-38836. Applications: Western Blotting
• Xing, J. et al. (1998) Nerve growth factor activates extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways to stimulate CREB serine 133 phosphorylation. Mol. Cell. Biol. 18, 1946-1955. Applications: Western Blot
• Maronde, E. et al. (1999) Transcription factors in neuroendocrine regulation: rhythmic changes in pCREB and ICER levels frame melatonin synthesis. J. Neurosci. 19, 3326-3336. Applications: ICC-ABC Western Blot
• Hu, B. R. et al. (1999) Persistent phosphorylation of cyclic AMP responsive element-binding protein and activating transcription factor-2 transcription factors following transient cerebral ischemia in rat brain. J. Neurosci. 89, 437-452. Applications: ICC-IF IHC-F (floating) Western Blot
• Pláteník, J. et al. (2005) J Neurochem 95, 1446-60. Applications: Western Blotting
• Sakaguchi, M. et al. (2008) Mol Biol Cell 19, 78-85. Applications: Western Blotting
• Almeida, L.E. et al. (2009) J Neurosci 29, 12702-10. Applications: Western Blotting
• Gehani, S.S. et al. (2010) Mol Cell 39, 886-900. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 1090 Phospho-CREB (Ser133) Blocking Peptide
• 9104 CREB (86B10) Mouse mAb
• 9193 CREB Control Cell Extracts
• 9196 Phospho-CREB (Ser133) (1B6) Mouse mAb
• 9197 CREB (48H2) Rabbit mAb
• 9198 Phospho-CREB (Ser133) (87G3) Rabbit mAb
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9187 Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor^® 488 Conjugate)

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.