Product Pathways - Neuroscience
CREB (48H2) Rabbit mAb #9197
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP IHC-P IHC-F IF-F IF-IC F | H M R Mk Dm | 43 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-F=Immunofluorescence (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Dm=D. melanogaster
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
CREB (48H2) Rabbit mAb detects endogenous levels of total CREB protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with GST-CREB full length fusion protein.
Western Blotting
Western blot analysis of extracts from SK-N-MC, NIH/3T3 and COS cells, using CREB (48H2) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human astrocytoma, using CREB (48H2) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using CREB (48H2) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using CREB (48H2) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse brain, using CREB (48H2) Rabbit mAb.
IHC-F (frozen)
Immunohistochemical analysis of frozen H1650 xenograft, showing nuclear localization using CREB (48H2) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of SK-N-MC cells, using CREB (48H2) Rb mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of SK-N-MC cells showing nuclear stain with CREB (48H2) Rabbit mAb (A, red) compared to an isotype control (B). Blue pseudocolor =DRAQ5® (fluorescent DNA dye).
IF-F
Confocal immunofluorescent analysis of mouse cerebellum labeled with CREB (48H2) Rabbit mAb (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor =DRAQ5® (fluorescent DNA dye).
Background
CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (7-9).
- Lonze, B.E. et al. (2002) Neuron 34, 371-385.
- Lee, M.M. et al. (1999) J. Neurosci. Res. 55, 702-712.
- Redmond, L. et al. (2002) Neuron 34, 999-1010.
- Dash, P.K. et al. (1990) Nature 345, 718-721.
- Yin, J.C. et al. (1994) Cell 79, 49-58.
- Guzowski, J.F. and McGaugh, J.L. (1997) Proc. Nat. Acad. Sci. USA 94, 2693-2698.
- Xing, J. et al. (1998) Mol. Cell. Biol. 18, 1946-1955.
- Ribar, T.J. et al. (2000) J. Neurosci. 20, RC107.
- Tan, Y. et al. (1996) EMBO J. 15, 4629-4642.
Application References
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Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9190 PhosphoPlus® CREB (Ser133) Antibody Kit
- 9191 Phospho-CREB (Ser133) Antibody
- 9193 CREB Control Cell Extracts
- 9196 Phospho-CREB (Ser133) (1B6) Mouse mAb
- 9104 CREB (86B10) Mouse mAb
- 9187 Phospho-CREB (Ser133) (87G3) Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 9198 Phospho-CREB (Ser133) (87G3) Rabbit mAb
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
