Product Pathways - MAPK Signaling
PhosphoPlus® p38 MAPK (Thr180/Tyr182) Antibody Kit #9210
|9210S||1 Kit (20 western blots)||---||In Stock||---|
|9210||carrier free and custom formulation / quantity||email request|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||Mol. Wt.||Isotype|
|Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511||200 µl||W, IP, IHC-P, IF-IC, F||H, M, R, Mk, Mi, Pg, Sc||Hm, C, Z, B||43 kDa||Rabbit IgG|
|p38 MAPK Antibody #9212||200 µl||W, IHC-P, IF-IC, F||H, M, R, Mk, GP||C||43 kDa||Rabbit|
|p38 MAPK Control Cell Extracts #9213||150 µl|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|Anti-biotin, HRP-linked Antibody #7075||250 µl||Goat|
|Biotinylated Protein Ladder Detection Pack #7727||250 µl|
|20X LumiGLO® Reagent and 20X Peroxide #7003||10 ml|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Mi=Mink, Pg=Pig, Sc=S. cerevisiae, GP=Guinea Pig
Western blot analysis of extracts from COS and 293 cells, untreated or UV-treated, using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 (upper) or p38 MAPK Antibody #9212 (lower).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p38 MAPK Antibody #9212.
Flow cytometric analysis of Jurkat cells, using p38 MAPK Antibody #9212 (blue) compared to a nonspecific negative control antibody (red).
The PhosphoPlus® p38 MAPK (Thr180/Tyr182) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of p38 MAPK at Thr180/Tyr182.
Specificity / Sensitivity
Phospho-p38 MAPK Antibody detects phosphorylated threonine 180 and tyrosine 182 of p38 MAPK but does not appreciably cross-react with the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human p38 MAPK (p38 MAP Kinase Antibody).
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).
SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
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- Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.
- Liu, F. et al. (2002) Mol. Endocrinol. 16, 419-434. Applications: Western Blotting.
- Legos, J. J. et al. (2001) . Brain Research 892, 70-77. Applications: Western Blotting.
- Watanabe, H. et al. (2001) . Journal of Biological Chemistry 276, 14466-14473. Applications: Western Blotting.
- De, A. K. et al. (2000) . Journal of Immunology 165, 3951-3958. Applications: Western Blotting.
- Lee, Y. W. et al. (2000) Cancer Lett. 156, 133-139. Applications: Western Blotting.
- Griego, S. D. et al. (2000) . Journal of Immunology 165, 5211-5220. Applications: Western Blotting.
- Novitskaya, V. et al. (2000) . Journal of Biological Chemistry , . Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
LumiGLO® is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.