Cell Signaling Technology

Product Pathways - MAPK Signaling

PhosphoPlus® p38 MAP Kinase (Thr180/Tyr182) Antibody Kit #9210

Kit Includes Quantity Applications Reactivity MW (kDa) Source
Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb # 9215 200 microliters W IF-IC F H M R Mk Mi Dr 43 Rabbit
p38 MAP Kinase Antibody # 9212 200 microliters W IHC-P IF-IC F H M R Mk 43 Rabbit
p38 MAP Kinase Control Cell Extracts # 9213 60 microliters
Anti-rabbit IgG, HRP-linked Antibody # 7074 100 microliters Goat
Anti-biotin, HRP-linked Antibody # 7075 200 microliters Goat
Biotinylated Protein Ladder Detection Pack # 7727 200 microliters
20X LumiGLO® Reagent and 20X Peroxide # 7003 10 milliliters

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Mi=Mink  Dr=Drosophila

Specificity / Sensitivity

Phospho-p38 MAP Kinase Antibody detects phosphorylated threonine 180 and tyrosine 182 of p38 MAP Kinase but does not appreciably cross-react with the corresponding phosphorylated forms of either p42/44 MAP Kinase or SAPK/JNK.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat, C6, NIH/3T3 and COS cells, untreated or treated as indicated, using Phospho-p38 MAP Kinase (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (upper) or p38 MAP Kinase Antibody #9212 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p38 MAP Kinase Antibody #9212.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, using p38 MAP Kinase Antibody #9212 (blue) compared to a nonspecific negative control antibody (red).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or anisomycin-treated (green), using Phospho-p38 MAP Kinase (Thr180/Tyr182) (3D7) Rabbit mAb #9215 compared to a nonspecific negative control antibody (red).

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Thr180/Tyr182 of human p38 MAP kinase. Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide derived from the sequence of human p38 MAP kinase (p38 MAP Kinase Antibody).

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as ERK6 or SAPK3) and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).

  1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  2. Han, J. et al. (1994) Science 265, 808-811.
  3. Lee, J.C. et al. (1994) Nature 372, 739-746.
  4. Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
  5. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
  6. Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
  7. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
  8. Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.

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