Product Pathways - MAPK Signaling
PhosphoPlus® p38 MAP Kinase (Thr180/Tyr182) Antibody Kit #9210
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|---|---|
| Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb # 9215 | 200 microliters | W IF-IC F | H M R Mk Mi Dr | 43 | Rabbit |
| p38 MAP Kinase Antibody # 9212 | 200 microliters | W IHC-P IF-IC F | H M R Mk | 43 | Rabbit |
| p38 MAP Kinase Control Cell Extracts # 9213 | 60 microliters | ||||
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 100 microliters | Goat | |||
| Anti-biotin, HRP-linked Antibody # 7075 | 200 microliters | Goat | |||
| Biotinylated Protein Ladder Detection Pack # 7727 | 200 microliters | ||||
| 20X LumiGLO® Reagent and 20X Peroxide # 7003 | 10 milliliters |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Mi=Mink
Dr=Drosophila
Specificity / Sensitivity
Phospho-p38 MAP Kinase Antibody detects phosphorylated threonine 180 and tyrosine 182 of p38 MAP Kinase but does not appreciably cross-react with the corresponding phosphorylated forms of either p42/44 MAP Kinase or SAPK/JNK.
Western Blotting
Western blot analysis of extracts from Jurkat, C6, NIH/3T3 and COS cells, untreated or treated as indicated, using Phospho-p38 MAP Kinase (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (upper) or p38 MAP Kinase Antibody #9212 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p38 MAP Kinase Antibody #9212.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using p38 MAP Kinase Antibody #9212 (blue) compared to a nonspecific negative control antibody (red).
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Thr180/Tyr182 of human p38 MAP kinase. Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide derived from the sequence of human p38 MAP kinase (p38 MAP Kinase Antibody).
Background
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as ERK6 or SAPK3) and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).
- Rouse, J. et al. (1994) Cell 78, 1027-1037.
- Han, J. et al. (1994) Science 265, 808-811.
- Lee, J.C. et al. (1994) Nature 372, 739-746.
- Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
- Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
- Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
- Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
- Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
Application References
- De, A. K. et al. (2000) Exaggerated human monocyte IL-10 concomitant to minimal TNF-alpha induction by heat-shock protein 27 (Hsp27) suggests Hsp27 is primarily an antiinflammatory stimulus [In ProcessCitation]. Journal of Immunology 165, 3951-3958. This article references the use of PhosphoPlus® p38 MAP Kinase (Thr180/Tyr182) Antibody Kit in the following applications: Western Blotting
- Legos, J. J. et al. (2001) SB 239063, a novel p38 inhibitor, attenuates early neuronal injury following ischemia. Brain Research 892, 70-77. This article references the use of PhosphoPlus® p38 MAP Kinase (Thr180/Tyr182) Antibody Kit in the following applications: Western Blotting
- Liu, F. et al. (2002) GnRH activates ERK1/2 leading to the induction of c-fos and LHbeta protein expression in LbetaT2 cells. Mol. Endocrinol. 16, 419-434. This article references the use of PhosphoPlus® p38 MAP Kinase (Thr180/Tyr182) Antibody Kit in the following applications: Western Blotting
- Lee, Y. W. et al. (2000) 2-Amino-3-methylimidazo[4,5-f]quinoline inhibits nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 cells by blocking p38 kinase activation. Cancer Lett. 156, 133-139. This article references the use of PhosphoPlus® p38 MAP Kinase (Thr180/Tyr182) Antibody Kit in the following applications: Western Blotting
- Griego, S. D. et al. (2000) Role of p38 mitogen-activated protein kinase in rhinovirus-induced cytokine production by bronchial epithelial cells[In Process Citation]. Journal of Immunology 165, 5211-5220. This article references the use of PhosphoPlus® p38 MAP Kinase (Thr180/Tyr182) Antibody Kit in the following applications: Western Blotting
- Novitskaya, V. et al. (2000) Oligomeric forms of the metastasis related Mts1(S100A4) protein, stimulate neuronal differentiation in cultures of rat hippocampal neurons. Journal of Biological Chemistry , . This article references the use of PhosphoPlus® p38 MAP Kinase (Thr180/Tyr182) Antibody Kit in the following applications: Western Blotting
- Watanabe, H. et al. (2001) Transcriptional Cross-talk between Smad, ERK1/2, andp38 Mitogen-activated Protein Kinase Pathways Regulates Transforming Growth Factor-beta -induced Aggrecan Gene Expression in ChondrogenicATDC5 Cells. Journal of Biological Chemistry 276, 14466-14473. This article references the use of PhosphoPlus® p38 MAP Kinase (Thr180/Tyr182) Antibody Kit in the following applications: Western Blotting
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- 9213 p38 MAP Kinase Control Cell Extracts
- 9214 p38δ MAP Kinase Antibody
- 9215 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb
- 9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb
- 9217 p38α MAP Kinase (5F11) Mouse mAb
- 9218 p38α MAP Kinase Antibody
- 9219 Immobilized Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb
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