Cell Signaling Technology

Product Pathways - MAPK Signaling

PhosphoPlus® p38 MAPK (Thr180/Tyr182) Antibody Kit #9210

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP™ Rabbit mAb # 4511 200 microliters W IP IHC-P IF-IC F H M R Mk Sc (Pg) (B) (Z) 43 Rabbit IgG
p38 MAPK Antibody # 9212 200 microliters W IHC-P IF-IC F H M R Mk GP 43 Rabbit
p38 MAPK Control Cell Extracts # 9213 60 microliters
Anti-rabbit IgG, HRP-linked Antibody # 7074 100 microliters Goat
Anti-biotin, HRP-linked Antibody # 7075 200 microliters Goat
Biotinylated Protein Ladder Detection Pack # 7727 200 microliters
20X LumiGLO® Reagent and 20X Peroxide # 7003 10 milliliters

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Pg=Pig  Sc=S. cerevisiae  B=Bovine  GP=Guinea Pig  Z=Zebrafish

Specificity / Sensitivity

Phospho-p38 MAPK Antibody detects phosphorylated threonine 180 and tyrosine 182 of p38 MAPK but does not appreciably cross-react with the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS and 293 cells, untreated or UV-treated, using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP™ Rabbit mAb #4511 (upper) or p38 MAPK Antibody #9212 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p38 MAPK Antibody #9212.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, using p38 MAPK Antibody #9212 (blue) compared to a nonspecific negative control antibody (red).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or anisomycin-treated (green), using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 compared to a nonspecific negative control antibody (red).

Description

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human p38 MAPK (p38 MAP Kinase Antibody).

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as ERK6 or SAPK3) and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).

  1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  2. Han, J. et al. (1994) Science 265, 808-811.
  3. Lee, J.C. et al. (1994) Nature 372, 739-746.
  4. Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
  5. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
  6. Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
  7. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
  8. Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.

Application References

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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