Product Pathways - MAPK Signaling

p38 MAP Kinase Antibody #9212

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IHC-P IF-IC F	H M R Mk Pg	Endogenous	43	Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Pg=Pig
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

p38 MAP Kinase Antibody detects endogenous levels of total p38α, -β or -γ MAP kinase protein. This antibody does not recognize p38δ, JNK/SAPK or p44/42 MAP kinase.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human p38 MAP kinase. Antibodies are purified by protein A and peptide affinity chromatography.

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as ERK6 or SAPK3) and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).

1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
2. Han, J. et al. (1994) Science 265, 808-811.
3. Lee, J.C. et al. (1994) Nature 372, 739-746.
4. Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
5. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
6. Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
7. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
8. Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.

Application References

• Matsumoto, M. et al. (2000) Involvement of p38 mitogen-activated protein kinase signaling pathway in osteoclastogenesis mediated by receptor activator of NF-kappa B ligand (RANKL). J. Biol. Chem. 275, 31155-31161. This article references the use of p38 MAP Kinase Antibody in the following applications: Western Blotting
• Chen, C. H. et al. (2000) Cyclic AMP activates p38 mitogen-activated protein kinase in Th2 cells: phosphorylation of GATA-3 and stimulation of Th2 cytokine gene expression. J. Immunol. 165, 5597-5605. This article references the use of p38 MAP Kinase Antibody in the following applications: Western Blotting
• Mudgett, J. S. et al. (2000) Essential role for p38alpha mitogen-activated protein kinase in placental angiogenesis. Proc. Natl. Acad. Sci. USA 97, 10454-10459. This article references the use of p38 MAP Kinase Antibody in the following applications: IHC-P (paraffin) Western Blotting
• Rossi, A. et al. (2000) Anti-inflammatory cyclopentenone prostaglandins are direct inhibitors of IkappaB kinase. Nature 403, 103-108. This article references the use of p38 MAP Kinase Antibody in the following applications: IP Western Blotting
• Zhuang, S. et al. (2000) p38 mitogen-activated protein kinase mediates bid cleavage, mitochondrial dysfunction, and caspase-3 activation during apoptosis induced by singlet oxygen but not by hydrogen peroxide. J. Biol. Chem. 275, 25939-25948. This article references the use of p38 MAP Kinase Antibody in the following applications: Western Blotting
• Hsu, J.T. et al. (2007) Am J Physiol Heart Circ Physiol 292, H2982-7. This article references the use of p38 MAP Kinase Antibody in the following applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 9210 PhosphoPlus® p38 MAP Kinase (Thr180/Tyr182) Antibody Kit
• 9211 Phospho-p38 MAPK (Thr180/Tyr182) Antibody
• 9213 p38 MAP Kinase Control Cell Extracts
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9214 p38δ MAP Kinase Antibody
• 9218 p38α MAP Kinase Antibody
• 2371 p38α MAP Kinase (7D6) Rabbit mAb
• 2307 p38γ MAP Kinase Antibody
• 9215 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb
• 9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb
• 9217 p38α MAP Kinase (5F11) Mouse mAb
• 9219 Immobilized Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb
• 9228 p38α MAP Kinase (L53F8) Mouse mAb
• 2308 p38δ MAP Kinase (10A8) Rabbit mAb
• 4631 Phospho-p38 MAPK (Thr180/Tyr182)(12F8) Rabbit mAb (IHC Preferred)

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.