Product Pathways - MAPK Signaling
p38 MAPK Antibody #9212
|9212L||600 µl (60 western blots)||---||In Stock||---|
|9212S||200 µl (20 western blots)||---||In Stock||---|
|9212||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey, Guinea Pig||Endogenous||43||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Chicken.
Specificity / Sensitivity
p38 MAP Kinase Antibody detects endogenous levels of total p38α, -β or -γ MAPK protein. This antibody does not recognize p38δ, JNK/SAPK or p44/42 MAPK.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human p38 MAPK. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® p38 MAPK siRNA I #6564 (+) or SignalSilence® p38 MAPK siRNA II (+), using p38 MAPK Antibody #9212 and α-Tubulin (11H10) Rabbit mAb #2125. The p38 MAPK antibody confirms silencing of p38 MAPK expression while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of p38 MAPK siRNA.
Western blot analysis of extracts from C6 cells, untreated or anisomycin-treated, and NIH/3T3 cells, untreated or UV-treated, using phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211 (upper) or p38 MAPK Antibody (lower).
Western blot analysis of extracts from NIH/3T3 cells, untreated or UV-treated for the indicated times, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211 (upper) or p38 MAPK Antibody (lower).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p38 MAPK Antibody.
Flow cytometric analysis of Jurkat cells, using p38 MAPK Antibody (blue) compared to a nonspecific negative control antibody (red).
Confocal immunofluorescent images of HeLa cells-/+ UV light labeled with #9212 p38 MAPK (green) exhibiting cytoplasmic localization in untreated cells (left) and nuclear localization in treated cells (right). Red = Actin filaments (phalloidin).
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).
SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
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- Miyaji, M. et al. (2009) Proc Natl Acad Sci U S A 106, 14502-7. Applications: Western Blotting.
- Hsu, J.T. et al. (2007) Am J Physiol Heart Circ Physiol 292, H2982-7. Applications: Western Blotting.
- Kojima, R. et al. (2004) Biochem J 380, 783-94. Applications: Western Blotting.
- Matsumoto, M. et al. (2004) J Biol Chem 279, 45969-79. Applications: IF-IC (In Cells).
- Matsumoto, M. et al. (2000) J. Biol. Chem. 275, 31155-31161. Applications: Western Blotting.
- Chen, C. H. et al. (2000) J. Immunol. 165, 5597-5605. Applications: Western Blotting.
- Mudgett, J. S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10454-10459. Applications: Western Blotting, IHC-P (paraffin).
- Rossi, A. et al. (2000) . Nature 403, 103-108. Applications: Western Blotting.
- Zhuang, S. et al. (2000) J. Biol. Chem. 275, 25939-25948. Applications: Western Blotting.
- Rohani, M.G. et al. (2010) BMC Immunol 11, 53. Applications: Western Blotting.
- Gehani, S.S. et al. (2010) Mol Cell 39, 886-900. Applications: Western Blotting.
- Lee, W.H. et al. (2011) J Biol Chem 286, 21577-87. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
SignalStain® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.