Product Pathways - MAPK Signaling
p38 MAPK Control Cell Extracts #9213
|9213S||150 µl (10 western blots)||---||In Stock||---|
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Nonphosphorylated p38 MAPK Control Cell Extracts: Total extracts from C-6 glioma cells prepared without anisomycin treatment to serve as a negative control. Supplied in SDS Sample Buffer.
Phosphorylated p38 MAPK Control Cell Extracts: Total extracts from C-6 glioma cells prepared with anisomycin treatment to serve as a positive control. Supplied in SDS Sample Buffer.
Western Blots: For controls, CST recommends using 15 µl of phosphorylated and nonphosphorylated p38 MAP Kinase Control Cell Extracts. Boil for 2 minutes prior to use.
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).
SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
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- Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
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- Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
- Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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