Product Pathways - MAPK Signaling
Phospho-ATF-2 (Thr71) Antibody #9221
|9221L||300 µl (30 western blots)||---||In Stock||---|
|9221S||100 µl (10 western blots)||---||In Stock||---|
|9221||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||70||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
Phospho-ATF-2 (Thr71) Antibody detects endogenous levels of ATF-2 only when phosphorylated at threonine 71. This antibody does not cross-react with phosphorylated c-Jun, CREB or other transcription factors. It recognizes both Thr69/Thr71 dually phosphorylated ATF-2 and Thr71 singly phosphorylated ATF-2 equally well.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF2. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from HeLa, C6 and NIH/3T3 cells, untreated or anisomycin-treated, using Phospho-ATF-2 (Thr71) Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma showing nuclear localization, using Phospho-ATF-2 (Thr71) Antibody .
Immunohistchemical analysis of paraffin-embedded human colon carcinoma using Phospho-ATF-2 (Thr71) Antibody.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-ATF-2 (Thr71) Antibody.
Immunhistochemical analysis of frozen H1650 xenograft using Phospho-ATF-2 (Thr71) Antibody.
Flow cytometric analysis of Jurkat cells, untreated (blue) or Anisomycin-treated (green), using Phospho-ATF-2 (Thr71) Antibody compared to a nonspecific negative control antibody (red).
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
- deRuiter, N. D. et al. (2000) . Mol. Cell. Biol. 20, 8480-8488. Applications: Western Blotting.
- Hocevar, B. A. et al. (1999) . EMBO J. 18, 1345-1356. Applications: Western Blotting.
- Marinissen, M. J. et al. (2001) . Genes Dev. 15, 535-553. Applications: Western Blotting.
- Ouwens, D. M. et al. (2002) . EMBO J. 21, 3782-3793. Applications: Western Blotting.
- Suh, Y. et al. (2000) . Cancer Res. 60, 5067-5073. Applications: Western Blotting.
- Zhang, S. and Kaplan, M.H. (2000) . J. Immunol. 165, 1374-1380. Applications: Western Blotting.
- Hou, N. et al. (2008) Endocrinology 149, 1654-65. Applications: Western Blotting.
- Ogino, T. et al. (2009) Leuk Res 33, 151-8. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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