Product Pathways - MAPK Signaling
Phospho-ATF-2 (Thr69/71) Antibody #9225
|9225S||100 µl (10 western blots)||---||In Stock||---|
|9225||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||70||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
Phospho-ATF-2 (Thr69/71) Antibody detects endogenous levels of ATF-2 only when dually phosphorylated at both threonine 69 and threonine 71. It does not recognize ATF-2 singly phosphorylated at either threonine 69 or threonine 71.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr69 and Thr71 of human ATF-2. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells, untreated or UV-treated (lanes 1 and 2), NIH/3T3 cells, untreated or anisomycin-treated (lanes 3 and 4), and C6 cells, untreated or anisomycin-treated (lanes 5 and 6), using Phospho-ATF-2 (Thr69/71) Antibody.
Immunohistochemical analysis of paraffin-embedded human colon carcioma using Phospho-ATF2 (Thr69/71) Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-ATF-2 (Thr69/71) Antibody.
Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or anisomycin treated (right), using Phospho-ATF-2 (Thr69/71) Antibody.
Immunohistochemical analysis of paraffin-embedded human lung carcioma, control (left) or lambda phosphatase-treated (right), using Phopsho-ATF2 (Thr69/71) Antibody.
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
- Alsayed, Y. et al. (2000) J. Biol. Chem. 276, 4012-4019. Applications: Western Blotting.
- Goh, K. C. et al. (1999) EMBO J. 18, 5601-5608. Applications: Western Blotting.
- Hartmann, G. and Krieg, A.M. (2000) J. Immunol. 164, 944-953. Applications: Western Blotting.
- Ouwens, D. M. et al. (2002) . EMBO Journal 21, 3782-3793. Applications: Western Blotting.
- Sellers, L. A. et al. (2000) Mol. Cell. Biol. 20, 5974-5985. Applications: Western Blotting.
- Zhong, S. P. et al. (2000) J. Biol. Chem. 275, 20980-20984. Applications: Western Blotting.
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