Cell Signaling Technology

Product Pathways - MAPK Signaling

PhosphoPlus® MKK3/MKK6 (Ser189/207) Antibody Kit #9230

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody #9231 100 µl W H M R 40 Phospho-MKK3. 41 Phospho-MKK6. Rabbit
MKK3 Antibody #5674 100 µl W H M R Mk 40 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat
Anti-biotin, HRP-linked Antibody #7075 100 µl Goat
20X LumiGLO® Reagent and 20X Peroxide #7003 5 ml each
Biotinylated Protein Ladder Detection Pack #7727 100 µl
MKK3/MKK6 Control Cell Extracts #9233 200 µl

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Phospho-MKK3/MKK6 (Ser189/207) Antibody detects endogenous levels of MKK3/MKK6 only when phosphorylated at Ser189/207. The antibody does not recognize the corresponding phosphorylated residues of MEK1, MEK2 or MKK4/SEK1. MKK3 Antibody detects endogenous levels of total MKK3 protein. This antibody does not cross-react with MEK1, MEK2, MKK4 or MKK6.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MKK3 Antibody #5674.

Western Blotting

Western Blotting

Western analysis of anisomycin-treated NIH/3T3 cell extracts using Phospho-MKK3/MKK6 and control MKK3 Antibodies.

Description

The PhosphoPlus® MKK3/MKK6 (Ser189/207) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of MKK3/MKK6 at Ser189/207.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser189/207 of human MKK3. Antibodies are purified by protein A and peptide affinity chromatography. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human MKK3. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 (2) and Ser207 and Thr211 on MKK6 (4,5).

  1. Derijard, B. et al. (1995) Science 267, 682-685.
  2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
  3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
  4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
  5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

Application References

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Protocols

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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