Product Pathways - MAPK Signaling
Phospho-MKK3/MKK6 (Ser189/207) Antibody #9231
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IP | H M R | 40 Phospho-MKK3. 41 Phospho-MKK6. | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-MKK3/MKK6 (Ser189/207) Antibody detects endogenous levels of MKK3/MKK6 only when phosphorylated at Ser189/207. The antibody does not recognize the corresponding phosphorylated residues of MEK1, MEK2 or MKK4/SEK1.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues around Ser189/207 of human MKK3. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or anisomycin-treated for the indicated times, using Phospho-MKK3/MKK6 (Ser189/207) Antibody (upper) or MKK3 Antibody #9232 (lower).
Background
MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site Thr-Gly-Tyr but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation of serine and threonine residues at sites Ser189 and Thr193 for MKK3 (2) and Ser207 and Thr211 for MKK6 (4,5).
- Derijard, B. et al. (1995) Science 267, 682-685.
- Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
- Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
- Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
- Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.
Application References
- Boppart, M. D. et al. (2000) Marathon running transiently increases c-Jun NH2-terminal kinase and p38 activities in human skeletal muscle. J. Cell. Physiol. 526, 663-669. This article references the use of Phospho-MKK3/MKK6 (Ser189/207) Antibody in the following applications: Western Blotting
- Browning, D. D. et al. (1999) Activation of p38 mitogen-activated protein kinase by lipopolysaccharide in human neutrophils requires nitricoxide-dependent cGMP accumulation. J. Biol. Chem. 274, 537-542. This article references the use of Phospho-MKK3/MKK6 (Ser189/207) Antibody in the following applications: Western Blotting
- Zhang, S. and Kaplan, M.H. (2000) The p38 mitogen-activated protein kinase is required for IL-12-induced IFN-gamma expression. J. Immunol. 165, 1374-1380. This article references the use of Phospho-MKK3/MKK6 (Ser189/207) Antibody in the following applications: Western Blotting
- Banerjee, S. et al. (2002) Murine coronavirus replication-induced p38 mitogen-activated protein kinase activation promotes interleukin-6 production and virus replication in cultured cells. J. Virol. 76, 5937-5948. This article references the use of Phospho-MKK3/MKK6 (Ser189/207) Antibody in the following applications: Western Blotting
- Kuemmerle, J.F. and Zhou, H. (2002) Insulin-like growth factor-binding protein-5 (IGFBP-5) stimulates growth and IGF-I secretion in human intestinal smooth muscle by Ras-dependent activation of p38 MAP kinase and Erk1/2 pathways. J. Biol. Chem. 277, 20563-20571. This article references the use of Phospho-MKK3/MKK6 (Ser189/207) Antibody in the following applications: Western Blotting
- Mita, H. et al. (2002) Regulation of MTK1/MEKK4 kinase activity by its N-terminal autoinhibitory domain and GADD45 binding. Mol. Cell. Biol. 22, 4544-4555. This article references the use of Phospho-MKK3/MKK6 (Ser189/207) Antibody in the following applications: Western Blotting
- Tikhomirov, O. and Carpenter, G. (2004) Ligand-induced, p38-dependent apoptosis in cells expressing high levels of epidermal growth factor receptor and ErbB-2. J. Biol. Chem. 279, 12988-12996. This article references the use of Phospho-MKK3/MKK6 (Ser189/207) Antibody in the following applications: Western Blotting
- DeWindt, L. J. et al. (2000) Calcineurin promotes protein kinase C andc-Jun NH2-terminal kinase activation in the heart. J. Biol. Chem. 275, 13571-13579. This article references the use of Phospho-MKK3/MKK6 (Ser189/207) Antibody in the following applications: Western Blotting
- Takekawa, M. et al. (2000) p53-inducible Wip1 phosphatase mediates a negative feedback regulation of p38 MAPK-p53 signaling in response to UV radiation. EMBO 19, 6517-6526. This article references the use of Phospho-MKK3/MKK6 (Ser189/207) Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9230 PhosphoPlus® MKK3/MKK6 (Ser189/207) Antibody Kit
- 9232 MKK3 Antibody
- 9233 MKK3/MKK6 Control Cell Extracts
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 9238 MKK3b Antibody
- 9236 Phospho-MKK3/MKK6 (Ser189/207) (22A8) Rabbit mAb
- 9264 MKK6 Antibody
