Product Pathways - MAPK Signaling

Phospho-MKK3 (Ser189)/MKK6 (Ser 207) Antibody #9231

supporting products
compare antibodies
control extracts

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W	H M R	Endogenous	40 Phospho-MKK3. 41 Phospho-MKK6.	Rabbit

Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by Western blot.

Protocols

9231:: Western Blotting

Specificity / Sensitivity

Phospho-MKK3/MKK6 (Ser189/207) Antibody detects endogenous levels of MKK3/MKK6 only when phosphorylated at Ser189/207. The antibody does not recognize the corresponding phosphorylated residues of MEK1, MEK2 or MKK4/SEK1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser189/207 of human MKK3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or anisomycin-treated for the indicated times, using Phospho-MKK3/MKK6 (Ser189/207) Antibody (upper) or MKK3 Antibody #9232 (lower).

Background

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site Thr-Gly-Tyr but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation of serine and threonine residues at sites Ser189 and Thr193 for MKK3 (2) and Ser207 and Thr211 for MKK6 (4,5).

Application References

Boppart, M. D. et al. (2000) Marathon running transiently increases c-Jun NH2-terminal kinase and p38 activities in human skeletal muscle. J. Cell. Physiol. 526, 663-669. Applications: Western Blotting
Browning, D. D. et al. (1999) Activation of p38 mitogen-activated protein kinase by lipopolysaccharide in human neutrophils requires nitricoxide-dependent cGMP accumulation. J. Biol. Chem. 274, 537-542. Applications: Western Blotting
Zhang, S. and Kaplan, M.H. (2000) The p38 mitogen-activated protein kinase is required for IL-12-induced IFN-gamma expression. J. Immunol. 165, 1374-1380. Applications: Western Blotting
Banerjee, S. et al. (2002) Murine coronavirus replication-induced p38 mitogen-activated protein kinase activation promotes interleukin-6 production and virus replication in cultured cells. J. Virol. 76, 5937-5948. Applications: Western Blotting
Kuemmerle, J.F. and Zhou, H. (2002) Insulin-like growth factor-binding protein-5 (IGFBP-5) stimulates growth and IGF-I secretion in human intestinal smooth muscle by Ras-dependent activation of p38 MAP kinase and Erk1/2 pathways. J. Biol. Chem. 277, 20563-20571. Applications: Western Blotting
Mita, H. et al. (2002) Regulation of MTK1/MEKK4 kinase activity by its N-terminal autoinhibitory domain and GADD45 binding. Mol. Cell. Biol. 22, 4544-4555. Applications: Western Blotting
Tikhomirov, O. and Carpenter, G. (2004) Ligand-induced, p38-dependent apoptosis in cells expressing high levels of epidermal growth factor receptor and ErbB-2. J. Biol. Chem. 279, 12988-12996. Applications: Western Blotting
DeWindt, L. J. et al. (2000) Calcineurin promotes protein kinase C andc-Jun NH2-terminal kinase activation in the heart. J. Biol. Chem. 275, 13571-13579. Applications: Western Blotting
Takekawa, M. et al. (2000) p53-inducible Wip1 phosphatase mediates a negative feedback regulation of p38 MAPK-p53 signaling in response to UV radiation. EMBO 19, 6517-6526. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.