Product Pathways - MAPK Signaling

MKK3 Antibody #9232

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W	H M R	Endogenous	40	Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

MKK3 Antibody detects endogenous levels of total MKK3 protein. This antibody does not cross-react with MEK1, MEK2, MKK4 or MKK6.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human MKK3. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site Thr-Gly-Tyr but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation of serine and threonine residues at sites Ser189 and Thr193 for MKK3 (2) and Ser207 and Thr211 for MKK6 (4,5).

1. Derijard, B. et al. (1995) Science 267, 682-685.
2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

Application References

• Takekawa, M. et al. (1998) Protein phosphatase 2Calpha inhibits the human stress-responsive p38 and JNK MAPK pathways. EMBO J. 17, 4744-4752. This article references the use of MKK3 Antibody in the following applications: Western Blotting
• Yu, L. et al. (2002) TGF-beta receptor-activated p38 MAP kinase mediates Smad-independent TGF-beta responses. EMBO J. 21, 3749-3759. This article references the use of MKK3 Antibody in the following applications: Western Blotting
• Banerjee, S. et al. (2002) Murine coronavirus replication-induced p38 mitogen-activated protein kinase activation promotes interleukin-6 production and virus replication in cultured cells. J. Virol. 76, 5937-5948. This article references the use of MKK3 Antibody in the following applications: Western Blotting
• Tibbles, L. A. et al. (1996) MLK-3 activates the SAPK/JNK and p38/RK pathways via SEK1 and MKK3/6. EMBO J. 15, 7026-7035. This article references the use of MKK3 Antibody in the following applications: Western Blotting
• Takekawa, M. et al. (1997) A human homolog of the yeast Ssk2/Ssk22 MAP kinase kinase kinases, MTK1, mediates stress-induced activation of p38 and JNK pathways. EMBO J. 16, 4973-4982. This article references the use of MKK3 Antibody in the following applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 9230 PhosphoPlus® MKK3/MKK6 (Ser189/207) Antibody Kit
• 9231 Phospho-MKK3/MKK6 (Ser189/207) Antibody
• 9233 MKK3/MKK6 Control Cell Extracts
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 9236 Phospho-MKK3/MKK6 (Ser189/207) (22A8) Rabbit mAb
• 9238 MKK3b Antibody
• 9264 MKK6 Antibody

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.