Cell Signaling Technology

Product Pathways - MAPK Signaling

MKK3/MKK6 Control Cell Extracts #9233

Description

Nonphosphorylated MKK3/MKK6 Control Cell Extracts: Total cell extracts from NIH/3T3 cells, prepared without treatment, serve as a negative control. Supplied in SDS Sample BufferPhosphorylated MKK3/MKK6 Control Cell Extracts: Total cell extracts from NIH/3T3 cells, prepared with UV light treatment, serve as a positive control. Supplied in SDS Sample Buffer.

Applications

As controls, CST recommends using 20 µl of phosphorylated and nonphosphorylated MKK3/MKK6 control cell extracts.

Background

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site Thr-Gly-Tyr but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation of serine and threonine residues at sites Ser189 and Thr193 for MKK3 (2) and Ser207 and Thr211 for MKK6 (4,5).

  1. Derijard, B. et al. (1995) Science 267, 682-685.
  2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
  3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
  4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
  5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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