Cell Signaling Technology

Product Pathways - MAPK Signaling

MKK3/MKK6 Control Cell Extracts #9233

Description

Nonphosphorylated MKK3/MKK6 Control Cell Extracts: Total cell extracts from NIH/3T3 cells, prepared without treatment, serve as a negative control. Supplied in SDS Sample Buffer.Phosphorylated MKK3/MKK6 Control Cell Extracts: Total cell extracts from NIH/3T3 cells, prepared with UV light treatment, serve as a positive control. Supplied in SDS Sample Buffer.

Western Blotting

Western Blotting

Western blot analysis of MKK3/6 Control Cell extracts using Phospho-MKK3 (Ser189)/MKK6 (Ser207) (22A8) Rabbit mAb #9236 (upper) and MKK3 Antibody #5674 (lower).

Applications

As controls, CST recommends using 20 µl of phosphorylated and nonphosphorylated MKK3/MKK6 control cell extracts.

Background

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 (2) and Ser207 and Thr211 on MKK6 (4,5).

  1. Derijard, B. et al. (1995) Science 267, 682-685.
  2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
  3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
  4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
  5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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