Product Pathways - MAPK Signaling
Phospho-MKK3 (Ser189)/MKK6 (Ser207) (22A8) Rabbit mAb #9236
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 40 P-MKK3, 41 P-MKK6 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Phospho-MKK3/MKK6 (Ser189/207) (22A8) Rabbit mAb detects endogenous levels of MKK3/MKK6 only when phosphorylated at serine189/207. The antibody does not recognize the corresponding phosphorylated residues of MEK1, MEK2 or MKK4/SEK1.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser189/207 of human MKK3.
Background
MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site Thr-Gly-Tyr but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation of serine and threonine residues at sites Ser189 and Thr193 for MKK3 (2) and Ser207 and Thr211 for MKK6 (4,5).
- Derijard, B. et al. (1995) Science 267, 682-685.
- Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
- Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
- Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
- Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.
Application References
- Giuliano, S. et al. (2010) Cancer Res 70, 3813-22. Applications: Western Blotting
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Companion Products
- 9230 PhosphoPlus® MKK3/MKK6 (Ser189/207) Antibody Kit
- 9231 Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody
- 9233 MKK3/MKK6 Control Cell Extracts
- 9238 MKK3b Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9264 MKK6 Antibody
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.